Preparation And Applications Of Phenylalanine And Its Silver-doped Modified Electrode

Chemically modified electrodes have been the most active frontier field of the electrochemistry and electroanalytical chemistry. Amino acid modified electrode is one of the important applications, which is being highly concerned by researchers because they have good sensitivity, selectivity and the special doping behavior as polymer modified electrode Catecholamine is a kind of containing the catecha and amino substances, and the content levels of them are related with diseases, such as sudden cardiac death,coronary heart, etc.Therefore, determining catecholamines with amino acid modified electrode has important value for clinical medicine and physiological function.First, we chose L-Phenylalanine as the modifying agents and poly (L-Phenylalaninc) modified glassy carbon electrode was then prepared by cyclic voltammetry. The electrochemical behaviors of EP,NE and DA were studied at the modified electrode.1. We first analyzed the factors that affect the prepare of the modified electrode.Optimal modification conditions of the modified electrode were discussed through comparing the current of the response,and the good reproducibility and stability of the modified electrode were verified by experiments.2. The voltammetric behavior of EP,NE,DA were determined on the optimal modified electrodes.The control mode of them with different concentrations were investigated on the modified electrode of PL-PHE/GCE,and we obtained the range of the concentration with which the anodic peak current of EP,NE,DA was in a good linear relationship,and the detection limit(S/N=3) that was two orders of magnitude lower than that of bare electrodeSecond, conductive polymer membrane having the good doping behavior can further improve the performance of the modified electrodes. The silver doped poly (L-phenylalanine) modified electrode was prepared by cyclic voltammetry. The voltammetric behavior of EP,NE DA and cyclic voltammetric method for the determination of them were studied on the silver doped poly (L-phenylalanine) modified electrode.1. We studied the optimal modification conditions of the silver doped poly (L-phenylalanine) modified electrode and the reproducibility and stability of the electrode were discussed through a series of experiments.2. The voltammetric behavior of EP,NE,DA were determined on the optimal modified electrodes. The control mode of them with different concentrations were investigated on the modified electrode of PL-PHE/GCE. We obtained the range of the concentration with which the anodic peak current of EP,NE,DA was in a good linear relationship,and the detection limit(S/N=3) that was three orders of magnitude lower than that of bare electrode and one order of magnitude lower than the poly (L-phenylalanine) modified electrode. At last, we found that a pair of sensitive redox peaks was exhibited in the presence of ascorbic acid (AA),uric acid (UA) and some other biological moleculars.Besides, the recovery rate was higher.In conclusion,that,the electrocatalysis of the silver doped poly (L-phenylalanine) modified electrode was more obvious than that of poly (L-phenylalanine) modified electrode,. The method has been applied to analysis of the injection sample, and the assay result was satisfactory...