Metal Sol Particles In The Sers Spectra Of Biomolecular Characterization

Since Surface-enhanced Raman scattering effect was discovered in the mid 1970's of the 20th century, research work about its mechanism and applications has been always being the focus of this field. Due to the ultra-high sensitivity of SERS, it can not only be used to characterize the molecular aggregates and single-molecule, but also be employed to analyze the molecular adsorption and orientation with respect to the metal surfaces. So in recent years it has been widely used in surface chemistry, electrochemistry, analytical chemistry, bioinorganic chemistry, metal corrosion and inhibition, as well as biological science etc. SERS spectroscopy based upon various kinds of metallic sol and the ordered assembly of nanoparticles, combines the advantages of both the SERS technique and the nanotechnology. It can be applied effectively to the study with biologically important molecules. At present, as for its performance, we should pay much more attention to its structural stability, spectroscopic reproducility and its SERS activity. This research work will enable us to perform more effectively our research work about interaction between biomolecules and substrates, as well as the relationships between molecular structural characteristics and their functions.Our research work includes the following aspects:(1) This paper briefly outlined normal Raman and SERS spectroscopy, the basic characteristics of the general application. In addition, it also briefed on the SERS applications about various metallic nanomaterials.(2) The present paper selects the dopamine, cysteamine for the representatives of biomolecules, and uses SERS technique combinied with the appropriate techniques respectively to characterize and analyze interaction between these biomolecules and core-shell nanoparticles. (3) By using chemical reduction and electrochemical deposition, we prepared a new type of Au NPs/ITO ordered assembly film. Field emission scanning microscopy and the SRES technique were employed to optimize the preparation conditions. Then we use this type of materials as the SERS substrate to carry out SERS investigation with azurin adsorption on such substrates.