The Electrochemical Characteristics Of The Interaction Of Glutamate And Adrenaline And Theoretical Studies

Protein is an essential element of all cells and organizational structure. It is the most important material basis in human life activities. There is no protein would be no life. The basic unit are composed of protein amino acids. As one of the many bioactive macromolecules of the body, amino acids are building cells, the basic materials for repair organizations. Epinephrine is an important material of catecholamine neurotransmitters for human and mammals, which controls the nerve system response to a series of biological and chemical processes. The research on molecular biological is to understand the interaction between biological systems and identify the specific role of the foundation. The study of the interaction between amino acid molecules and epinephrine are for the promotion of life sciences, pharmaceutical chemistry, physical chemistry. In this paper, we studied the influence of glycine, serine, glutamic acid, aspartic acid and phenylalanine on oxidation of epinephrine with AC impedance, and focused on the electrochemical behaviour of epinephrine after adding glutamic acid. At the same time, we assisted in quantum chemistry methods to carry out the theoretical study of hydrogen bonding complexes formed between glutamic acid and epinephrine.First of all, we study the properties of electron transfer of epinephrine after adding glycine, serine, glutamic acid, aspartic acid and phenylalanine in hydrochloric acid with AC impedance. The experimental results show that the five kinds of amino acids with different structures hinder electron transport capacity of epinephrine in the order:glycine 1.8, it is as a multi-step reaction mechanism, and in weak acid solution is in line with the mechanism of ECCECE. At this time it can produce five peaks, and there are two pairs of redox peaks. When the solution is neutral media, it only has four peaks, and there are two pairs of redox peaks. Finally, we do a theoretical study about the hydrogen bonding between epinephrine and glutamic acid. We optimized their configurations using the DFT-B3LYP method of quantum chemistry. The geometries of epinephrine and the stable complexes were respectively optimized to obtain structural parameters and binding energies. The calculation results could be used to explain some experimental phenomena better. The 1:1 hydrogen-bonded complexes of the Glu-Ep is fourteen in gas phase and six in solution phase. The formation of hydrogen bonds plays a protective effect on the hydroxy-adrenergic. It makes the adjacent hydroxyl (-OH) of the benzene ring remove H difficultly, reduces the energy system, increases the stability of epinephrine, inhibits the oxidation of the epinephrine to a certain extent and explains the phenomenon of the experiment combining structural parameters and energy.