Go Lysine-specific Methylase 1 (, Lsd1) In The Cell Function Of The Mitotic

In eukaryotes,dynamic methylation and demethylation-mediated chromatin remodeling and gene expression occurs throughout the cell cycle.Lysine specific demethylase 1(LSD1) is the first histone demethylase,acting on demethylation of mono-and di-methylated histone H3 on lysine 4(H3K4) and lysine 9(H3K9) and regulating target gene's transcription.We show here that LSD1 is hyperphosphorylated in nocodazole-synchronized cells.LSD1 localizes on centrosomes during interphase and spindle poles during mitosis.Inhibition of LSD1 expression by siRNA leads to abnormal chromosomal segregation in unperturbed mitosis,and is associated with decreased protein levels of MAD2 and BUBR1.LSD1 is a positive regulator of both BUBR1 and MAD2 promoters and maintains local monomethylation of H3K9.Our results suggest that LSD1 plays a role in chromosomal segregation during mitosis through transcriptional regulation of BUBR1 and MAD2.