| Fluorescence analysis is now used for a wide range of quantitative applications and offers the advantages of high sensitivity, rapidity, stability, simplicity and feasibility. The main purpose of this research is to find some new fluorescence systems and to sensitively detect some biologically active compounds.This thesis consists of two parts: review and research report. In the review section, the basic principles and characteristics are introduced, and the development of fluorescence analysis in pharmaceutical analysis and the interaction of small molecule with nucleic acids are reviewed. The research section contains three subunits, as follows:1. Fluorometric sensing of malachite green with calf thymus DNA as sensing materialA novel fluoremetric sensing of malachite green is proposed in this work. The native double-stranded calf thymus DNA was used as sensing material. In the presence of native double-stranded calf thymus DNA, malachite green could bind to DNA, which resulted in a strong fluorescence emission in this system. The fluorescent intensity was linear with malachite green concentration in the range of 3.6×10-10-1.8×10-7 g/mL and the limit of detection was 1.1×10-10 g/mL.2. Fluorometric sensing of berberine with G-rich DNA as sensing materialBerberine could interact with G-rich single stranded DNA to form G-quadruplex DNA structures, and the obtained supermolecule system showed strong fluorescence emission. Based on this phenomenon, a new fluorescence method for berberine was proposed. The effect of different variables such as pH, DNA concentration, temperature and time were investigated, and then the optimum conditions were established. The calibration graph was linear in the range of 6.0×10-9～4.0×10-7 g/mL of berberine. The detection limit was 1.8×10-9 g/mL.3. Simple and sensitive determination of dipyridamole with fluorescent methodThe fluorescence property of dipyridamole was studied in acidic, neutral and basic media. It was found that the strongest fluorescence intensity of dipyridamole was obtained in basic media. Based on the phenomena, a simple and sensitive fluorometric method for determination of dipyridamole was developed. The linear range of this method was 1.0×10-9～1.0×10-7g/mL, and the detection limit was 3.0×10-10g/mL. Furthermore, the fluorescence quantum yield of dipyridamole in various media was measured in order to explain the phenomena. |
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