Estrogen Receptor Beta (of Er¦Â) Expression In The Female Mandarin Vole Social Behavior And The Brain Regions Otr And Avpv1ar Of Impact

Animal social behavior,was controlled and regulated by centre nervous system and neuroendocrine system.Neuropeptide,sex hormone,a number of endocrine factors and environments where animal lives cooperate to affect social behavior of animals.Many experimental evidences indicate that E,OT and AVP acting in the brain appear to be critical for social discrimination.They could affect many social behaviors,such as social recognition,aggression, sexual behavior,partner preference,affinity behavior and individual behavior.Whether ERβcan regulate the OTRmRNA and AVPV1aRmRNA has not been reported.We preit that estrogen may show much regulation on the levels of OT and AVP messenger RNA.So,the present study will aim at the two problems:1,how E could affect some social behaviors in voles;2,Is there effects to OTR and AVPV1a expression by E receptor?we used monogamous female mandarin voles(Microtus mandarinus) as research objects. Combining antisense nucleotides technic with behavioral observation,we examined the correlative behavior after i.c.v,continuous injection of antisense nucleotides of estrogen receptor-β.Meanwhile, Combined antisense nucleotides technic with in situ hybridization,we investigated how ERβ, vasopressin,oxytocin and its receptors controlled complex social behaviors in female voles.1.Selected female adult mandarin voles were given i.c.v,continuous injections of antisense nucleotides of estrogen receptor-βrespectively in first day,third day and fifth day.After six hours, we examined aggression behavior during 10 minutes.The results were following:Compared with group of injection of scramble nucleotides,anisense group showed significant increase in frequency and total duration in aggression behavior.We suggested that antisense nucleotides of ERβinhibit the translation of ERβin mRNA levels.Furthermore,it blocked E to modulate aggression behavior in male voles.2.Selected female adult mandarin voles were given i.c.v,continuous injections of antisense nucleotides of estrogen receptor-βin first day,third day and fifth day.After six hours,we examined partner preference behavior during 60 minutes.The results were following:There were significant differences in visiting duration in mate's compartment,strange heterosexual vole's compartment and neutral compartment,and significant increase in the number of AVPV1RmRNA and OTRmRNA in BST,CE,LSD,PVN and ME,It is suggested that continuous injection of antisense nucleotides of ERβcan enhance social recognition,and can enhance the pair bond formation of male and female voles. 3.Selected female adult mandarin voles were given i.c.v,continuous injections of antisense nucleotides of estrogen receptor-βrespectively in first day,third day and fifth day.After six hours, we examined affinity behavior during 60 minutes.The results were following:There were significant enhance in amicable behavior and decrease in defensive behavior with its mate.At the same time,there were significant enhance in defensive behavior and decrease in investigating behavior with the strange heterosexual voles.It suggested that there were different nervous circuit, which female voles recognized socially its mate and stranger voles in the preference behavior examination.4.Selected female adult mandarin voles were given i.c.v,continuous injections of antisense nucleotides of estrogen receptor-βin first day,third day and fifth day.After six hours,OTR mRNA was examnined using in situ hybridization method in female voles.The results were following: There were significant differences in the BST,CE,ME,PVN and AH.Compared with injection of scramble nucleotides and injection of isotonic saline treatment,there were significant reduction in the number of OTR mRNA in AH,PVN and ME,and significant increase in the number of OTR mRNA in BST and CE Compared with injection of saline.These data suggested that the roles between antisense nucleotides of ERβand OTR affected social behavior in the female voles.It explained that ERβmodulated social behavior in female voles possibly through oxytocin and its receptor.5.Selected female adult mandarin voles were given i.c.v,continuous injections of antisense nucleotides of estrogen receptor-βin first day,third day and fifth day.After six hours, AVPV1RmRNA was examnined using in situ hybridization method in female voles.The results were following:There were significant differences in the BST,CE,PVN and LSD.Compared with injection of scramble nucleotides and injection of isotonic saline treatment,there were significant increase in the number of AVPV1RmRNA in BST,PVN and LSD,and significant reduction in the number of AVPV1R mRNA in CE.These data suggested that the roles between antisense nucleotides of ERβand AVPV1R affected social behavior in the female voles.Results from those conclusion that ERβ,OTR and AVPV1aR have nearly relation with female adult mandarin voles.ERβcan reduce the aggression behavior at a certain extent.The antisense nucleotides of estrogen receptorβcan enhance the preference of mandarin voles for mate or strange heterosexual vole,and strengthen the relation with mate in female adult mandarin voles.So,all behaviors were regulated by the nerve internal secretion mechanism.