Beet Reproductive Cloning,

The higher plants' life history is the mode of reproduction which appears alternately by repeatability and predictability. The plant's early embryonic development is developed by the cell division including the vital processes like zygote activation, polarity formation, morphogenesis and organogenesis, etc.The development of plant embryo is a very complicated process. It is not an independent action by only one gene, but a process controlled by plenty of genes. In exordium, I reviewed these genes and their expression features in every period of the embryo genesis in detail.In this study, the material of Beta vulgaris line M14 was used to genes which have important relationships with the reproductive development. We choose Beta vulgaris line M14 to be our experiment object, not only because Beta vulgaris are widely planted in China, what is more important, people obtained some precious subspecies in the long-term cultivating process, and accumulated plenty of genetic informations. The line M14 which we choose has an obvious characteristic of apomixes in cyto embryological observation cell embryology and genetics.In this study we use the method of homologous clone by the technique of Rapid Amplification of cDNA End (RACE). According to the homology sequence from other species, we designed degenerate primer, and gained MARB, full length of CK2 gene, with 3-RACE fragments and 5'-RACE fragments by 3'-RACE and 5'-RACE techniques.CK2,s full-length complete coding sequence is 1002bp (333 araino acid), and homologous alignment indicates that it is 64.22% homology with tobacco, 65.18 % homology with lily.MARB's, full-length complete coding sequence is 1719bp (572 amino acid), homologous alignment indicates that it is 61.26% homology with tomato, 67.20% homology with tobacco.To overcome the low expression abundance, we should enhance the used amount of cDNA template and with optimum conditions of PCR(including good quality cDNA as templates, the higher temperature as Tra, and so on) and repeat experiment multiple times. We should keep on studying the low expression abundance genes, and make a furthermore studying and verification.