The Cork Oak Seedlings And Cpdna Geographic Population Variability Study

Q.variabilis is one of the most important tree species in our country with multitude usages.We collected the seeds of 19 natural populations which distributed mainly in china,and made the initial research about seeds phenotype,seedling variation, and we studied the variation of populations by PCR-RFLP technique.The main results are as follows:(1)Seeds phenotype and seedling variations indicated that:The size of seeds(straight length,curved length,straight width,curved width),and the grain had significant variations between provenance and family constellation.The provenance variations of seedling ratio and seedling height were significant. Seeds phenotype appeared to have a second-degree parabola relation with the latitudes or longitude of the communities.There was significant positive correlation between seed size and germination rate.There was no correlation between single granule weight and seedling height, and there was no obviously law accompanied with latitudes or longitude.(2)The results of PCR-RFLP about 4 cpDNA fragments: There were fine polymorphism between the 19 populations, and 9 haplotypes observed.The haplotypes variation happened between populations, and there were faint variation among them.The Gst was 0.8488, and 1.512% genetic diversity among the populations. There were much more haplotypes in Anhui,Hubei,Shanxi which were the distribution center of Q.variabilis, and there were only 2 haplotypes in Jiangsu,Jiangxi,Fujian,Yunnan which were not the center. However these two haplotypes also existed in the distribution center. All the results indicated that: The distribution center of Q.variabilis was also the genetic diversity center. The existent distribution structure of Q.variabilis was probably from chuanxi located to the southern of Qinling Mountain to Dabieshan Mountain after the Quaternary period, and diffused to anywhere with each different adaptive form.