Of Raft Molecular Imprinting Technique Is A Preliminary Study

This dissertation consists of five chapters, and here include:(1) an overview of Molecular Imprinting Technology (MIT) and Reversible Addition-Fragmentation chain Transfer polymerization (RAFT);(2) the synthesis of Molecularly Imprinted Polymers (MIPs) nano-film hybrid silica gel and characters study on it;(3) the synthesis of surface imprinted core-shell nanoparticle and its application study;(4) the primary discussion on separation and extraction of active substance from marine microbial by RAFT-Molecular Imprinting Technology; and (5) conclusion and prospect of the research.Chapter one consists of two parts. In the first part, a brief introduction of MIT is given, topics discussed include the mechanisms, synthesis methods, characteristic properties and applications of MIPs. In the second part, a brief introduction of the current development of RAFT is summarized. The research objective and experimental plan of this dissertation is also presented.In chapter two, result on the synthesis and characterisation study of MIPs nano-film hybrid silica gel is described. The MIPs nano-film hybrid silica gel was prepared via Reversible Addition-Fragmentation chain Transfer polymerization (RAFT). The controllable nature of RAFT allows for the growth of MIP nano-film with adjustable thickness on the silica gel surface. Thus, it is possible to tailor the synthesis of either thicker MIP nano-films for capacity improvement, or thinner MIP nano-films for efficiency improvement. The results extend the synthetic method of MIPs and the results are significant for the preparation of molecular imprinting solid phase extraction materials and chromatographic media.In chapter three, the synthesis and application of the surface imprinted core-shell nanoparticle is discussed. The surface imprinted core-shell nanoparticle was synthesized through RAFT and its potential use as the recognition element in competitive fluorescent binding assay was demonstrated. The method can also be extended to other core nanoparticles with different geometries and chemistries, e.g. nano-magnetic fluid and nano-Au, to further expand scope of the application of MIPs in analytical chemistry.In chapter four, the separation and extraction of active substance from marine microbial by RAFT-Molecular Imprinting Technology is discussed. MIPs is synthesized in the presence of harmine, a structural analogues of norharman, by RAFT-Molecular Imprinting Technology. The resulting MIP was used as a chromatographic media for the separation and extraction of active substances from secondary metabolites of Pseudoalteromonas sp. NJ6-3-1.The result verified the possibility of the separation and extraction of active substance from marine microbial by RAFT-Molecular Imprinting Technology.In the final part of the dissertation, Chapter five, the significant innovation and findings in this dissertation are concluded, and the future prospect of this research field is described.