Rice Early Salt Stress In The Growth Regulation Of Dna Methylation

In this paper, we attempted to correlate the DNA methylation patterns induced by high salt pressure with rice seedling growth regulation. That is to say, what it might be when salinity causes DNA dynamic modification? We examined the phenotype in the seedling specific stage, with their physiological and biological appearances after 5-azac or cold pretreatment.The features of DNA mthyl-patterns in plant genome and stress signal pathway should be considered when we carry out the analysis. Descriping as following points: (1): there is a linear relationship with 5-Azac densities and the seedling development character. Although 5-Azac led to dwarfism and decadent color, it promoted seedling differentiation when the first leaf was stretching out. While, cold resulted in a delayed process.(2): when the differentiation came out, we checked the methyl levels in different conditions.including non stress, serials of 5-azac concentration pretreatment and cold interference. The results accompanied with whole experiment.(3): cell cycle arrest caused by salt stress and 5-Azac may reflect the DNA methylation regulation in some aspects. Whether it led to DNA damage or cuted off cell cycle signal cascades was not sure.(4): 5-Azac ingluenced chlorophyll content and composition. they were dependent on the concentration. Compared with the cold, non stress, high salt pressure groups, we discussed the methylation importance to nuclear and organelle genome. (5):5-Azac pretreatment signified different soluble protein categories in the electrophorysis results. We also certified 5-Azac almost has no influences on the protein density originated from salt stress .on the contrary, cold made the reaction actively.(6):a novel cis-element in antioxidative genes was chosed as a detector in EMSA, The signals differed greatly in the groups. More examination should be done to figure it out.