| Salt cress (Thettungiella halophila), a close relative of the model plant Arabidopsis thaliana, is an extremophile that is adapted to harsh saline environment. To mine salt tolerance genes from this species, we constructed an entry cDNA library from the salt cress plant treated with salt-stress by using a modified cDNA synthesis and an improved recombination-assisted cDNA library construction method that is completely free of manipulations involving restriction enzymes and DNA ligase. This cDNA library construction procedure is drastically simplified and the quality of cDNA library is improved. This entry cDNA library was subsequently shuttled into a destination binary vector pCB406 designed for plant transformation and expression via recombination-assisted cloning. The library is plant transformation-ready and is used to transform Arabidopsis in large-scale in order to create a large collection of transgenic lines for functional gene mining. |
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