Reorganization Of Production Of Cellulase Fungal Genome

Cellulose is the most abundant organic raw material in the world, and it is the only renewable resource that is available in large quantities. Cellulase can decompose the natural cellulose into cellulose molecular chain, cellobiose and glucose. It has great realistic meaning for human being to solve environment pollution, food shortage and energy crisis that using cellulase produced by microorganism transforms cellulose to energy, chemical and food.Molecular breeding inherited and improved crossbreeding; it accelerated the breeding-schedule by using new notion and method. The object of genome shuffling is the whole cell, it made cross breeding to mutation strain, and cause different strain's genome to recombine completely, the chance of various forward mutation concordances to a recon improves. Simultaneity, accumulative detrimental mutation was replaced by the sequence of wild type strain. Hence, improved recon was detected by high flux, and then entered the next genome shuffling; the forward mutation rate can be enhanced greatly.Therefore, using the Penicillium decumbens as original strain, which produces cellulase, this research subject prepared protoplasts and made recursive fusion of them to fulfill the recombination among cells, and then we selected a series of hybrids with the better quality than the parental strains by genome shuffling. We contrast the hybrids with the parental strains from the morphologic characteristics, cellulase activity and extra-cellular protein electrophoresis strips, thus make sure the relative relationship between the hybrids and parental strains, and interpret the exchange and separation of chromosome in the course of fusion.Take the strain M114 and Ju-A10 as examples; we studied the preparation and regeneration using the hypha and spores of Penicillium. The...