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Producing Strains Of Lipoprotein Lipase Screening, Culture Optimization, Purification And Basic Properties

Posted on:2007-01-18Degree:MasterType:Thesis
Country:ChinaCandidate:B YangFull Text:PDF
GTID:2190360185494050Subject:Biochemistry and Molecular Biology
Abstract/Summary:PDF Full Text Request
Lipoprotein lipase [LPL, EC 3.1.1.3] is a key enzyme to regulate the lipid fuel handing. It can detect lipid in serum quickly and precisely, which is been used to diagnose angiosclerosis and other lipid disorder diseases. With its higher sensibility and specificity, it has already applied to clinical diagnosis.A identified strain Candida rugosa, which could produce lipoprotein Lipase, was screened from 24 clones from institute of microbiology Chinese Academy of Sciences and ATCC.
Keywords/Search Tags:Lipoprotein lipase, Candida rugosa, screening, identification, Candida Rugosa, Fermentation, Optimization, Lipoprotein Lipase, DEAE-Sepharose F.F. chromatography, Phenyl Sepharose CL-4B chromatography, purification, Properties, K_m, Isoelectric point
PDF Full Text Request
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