Producing Strains Of Lipoprotein Lipase Screening, Culture Optimization, Purification And Basic Properties | Posted on:2007-01-18 | Degree:Master | Type:Thesis | Country:China | Candidate:B Yang | Full Text:PDF | GTID:2190360185494050 | Subject:Biochemistry and Molecular Biology | Abstract/Summary: | PDF Full Text Request | Lipoprotein lipase [LPL, EC 3.1.1.3] is a key enzyme to regulate the lipid fuel handing. It can detect lipid in serum quickly and precisely, which is been used to diagnose angiosclerosis and other lipid disorder diseases. With its higher sensibility and specificity, it has already applied to clinical diagnosis.A identified strain Candida rugosa, which could produce lipoprotein Lipase, was screened from 24 clones from institute of microbiology Chinese Academy of Sciences and ATCC. | Keywords/Search Tags: | Lipoprotein lipase, Candida rugosa, screening, identification, Candida Rugosa, Fermentation, Optimization, Lipoprotein Lipase, DEAE-Sepharose F.F. chromatography, Phenyl Sepharose CL-4B chromatography, purification, Properties, K_m, Isoelectric point | PDF Full Text Request | Related items |
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