Ap-1 Signaling Pathway Cell Model,

AP-1 (The activator protein 1), as a very important cell biosensor, can response to a plethora of external stimuli and regulate a wide range of cellular processes. It is a homo- or hetero-dimers composed by Fos , Jun , ATF and MAF protein family members and belongs to basic region-leucine zipper (bZIP) transcription factor family. Fos and Jun are the most common AP-1 proteins in mammalian cells with c-Fos and c-Jun dimmer being the main form due to its stability. AP-1 binds to TPA response elemet (TRE:5' >TGAC/GTCA<3' )and regulates the downstream genes expression in response to growth factors, cytokines, UV, stress, viral and bacterial infections and carcinogen etc.AP-1 which locat in the end of cascade of signal pathway is a bridge of external stimulus and cell function. The genes induced by AP-1 are diverse in multiple functions such as proliferation, differentiation, transformation, apoptosis , inflammation, survival or death , and immunity etc. Therefore, finding the positive stimulus of AP-1 will be beneficial to anti-tumor , anti-inflammation and anti-apoptosis et al, and still helpful to elucidate the mechemism of growth.Our work is aimed to construct a stable cell line of AP-1 signaling pathway to screen any stimulus activating the signal pathway and analyze the drug efficacy and specificity associated with AP-1 . The positive stimulus can be used as a drug candidate for further clinical trial. Our stable cell line was derived from human 293E cells with chromosomal integration of an anti-puromycin gene and a GFP reporter gene construct regulated by 8 copies of the AP-1 response element that we synthesized in the company. This cell line has strong response to the TPA induction when assayed by the GFP flurensence.The result presents the clonal cell line works well, and it is very...