.1. Peptide Chain Elongation Factor Expression Characteristics Of Lipid Metabolism Related Genes In Mice Liver Differentially Expressed Proteins

Peptide elongation factors(eEFlA) is the required protein factors in the process of protein biosynthesis, with kinds of effects, such as regulating protein biosynthesis and accelerating apoptosis, et.al. previous studies have shown that a developmental switch between eEF1A-1 and eEF1A-2 occurs in neurous and skeletal muscle, the mutant mice have development abnormity in the system of nerve, muscle and immunity, and died at 28 days of age, indicated that eEFlA-1 and eEFlA-2 have the parallel biological effects. And in the postnatal mice, eEFlA-2 play an important role in development and mature of the system of nerve and muscle. Western blotting and Immunofluorescence techniques have been employed for analyzing expression patterns as well as in situ localization of eEF1As in neurons of wild type and eEF1A-2 spontaneous mutant mice at differential development phases, and also discussed the connection of maintaining cell physiological function.During development the expression of eEF1A-2 gradually increase and absolutely replace the eEF1A-1 expression by 26 days of age after birth. The eEFlA-2 was absent in mutant mice and mutant mice exhibit muscle wasting, neurological impairment, immunological abnormalities, and tremors beginning at 21 days of age. And all died by 28 days of age after birth. The characteristic of eEF1A-1 expression in mutant mice is consistent with the wild mice. The expression level and space-time is not changed without eEF1A-2 gene in neurous, indicated that the function of regulating is not effected by the eEF1A-2 expression and the level, further more, proved that there is a eEF1A-1 expression regulatory mechanism independent of eEF1A-2. the mutant mice characterized by tremors and lethargy, occurs simultaneously with complete disappearance of eEF1A-1, and absence of eEF1A-2 proteins, from neurons, heart, and skeletal muscles. Thus, eEF1As is very important for the protein biosynthesis.Atherosclerosis is a kind of complex multifactorial disease. Many complicated factors interaction and interrelated biological processes contribute to atherogenesis. Study of its mechanisms can provide available preventive measures and clinical therapy strategies. At present study, apoE^-/-/LDLR^-/-/Lepr^db/db treble-gene mutant mice were established and used for study the mechanisms of polygenetic disfunction in dyslipidemia as well as atherogenesis. The relationship between treble-genetic mutation and hyperlipidemia/ hyperlipoprotein, following atherosclerotic pathological...