| During a long time, People have fought with annoying mold by some chemical preservative, like Sodium benzoate and Potassium sorbate. But investigation showed that chemical preservative were almost poisonous matter. It was harmful to one's health. People wanted to find a natural produce which is harmless to people and efficiently inhibiting from mold. But to our disappointment, there has been not a inspiring report on an perfect natural produce. Eutrema wasabi Maxim is a crucifer. Research men put most of their focus on its medicative function. Its value of inhibiting mold has been neglected all the while. There have not been detailed reports on wasabi's function of restrain mold in national and international investigation except for a short news come from a Japanese company. It is especially lack on this aspect nation-wide.This paper aimed to explore wasabi's value of inhibiting mold. We studied the inhibiting impact and the mechanism that wasabi's effective composition (SK) affected the indicated mold of GJB.Method:1. The conventional experiment of SK restraining mold was investigated. The effect of SK and several preservative on the indicated mold was compared, and the effect of temperature and PH on SK's inhibiting impact was measured by filter paper methed. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of SK were educed by MIC method. 2. The effects of SK on the spore germination ratio of the indicated mold was measured by microscope count method. 3. The effects of SK on mold's ratio of absorbing and utilizing reducing sugar and protein was studied by coomassie brilliant blue G250 and 3,5-dinitrosaliculic acid method. 4. The membrane damage of SK on the indicated mold was measured by conductance method, and the connection between the SK concentration and the membrane damage ratio was analyzed . 5. The effects of SK on several ATPase activity of the indicated mold was measured by ATPase testing-box. Then the effects of SK on ion transport efficiency and ion concentration stabilization of the mold could be analyzed. 6. The effect of SK on mitochondria respiration speed of the indicated mold was measured by Clark electrode method. 7. The effect of SK on SDHase activity of the indicated mold was measured by SDHase testing-box, and the effects of SK on tricarboxylic acid cycle and respiratory chain of the mold mitochondria were analyzed.Result:1. SK could significantly inhabit the indicated mold. Its effect was obviously better than which of the chemical preservative, like Sodium benzoate and Potassium sorbate. The five indicated mold have their respective minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of SK. It was 0.15mg/mL and 0.20mg/mL for Aspergillus fla vus, A.niger, A. versicolor and P. funiculosum. It was O.lOmg/mLand 0.15mg/L for C. globusum. SK was the most effective in the Temperature of 18°C—60'C and PH 9.0, besides it was stably in high temperature.2. Observation of microscope showed that SK inhibited the mold spore tarnally. The spore almost could not bourge as a result of SK.3. The mold's ratio of absorbing and utilizing reducing sugar and protein have dropped respectively by 55% and 44% at least. It showed that SK damaged the matter transport.4. The damnification of membrane obviously ascend along with the concentration of SK. It was deteriorative with the concentration of 0.10 mg/mL—0.25 mg/mL.5. SK reduced the NaT-ATPase, Mg"-ATPase and Ca"-ATPase respective activity by 85.69 %,43.61% and 96.95%. It showed that SK effected on ion transport efficiency and ion concentration stabilization of the mold.6. SK reduced the mitochondria respiration speed with pyruxic acid, a -keto-glutaric acid and succinic acid as substrate by 38.72%, 54.48% and 63.79%.7. SK reduced the SDHase activity by 59.15%. It indicated that SK related with the tricarboxylic acid cycle and respiratory chain of the mold mitochondria. |
PDF Full Text Request