Blue Light Regulation Of Arabidopsis Auxin Biosynthesis, Polar Transport Mechanism And The Random Of The Gfp :: Cdna Fusion Gene Was Inserted Into The Cloning And Functional Analysis

This paper has two parts: (1)In this report ,colombian wild type col-4 and blue light receptor mutant cry1-304 , cry2 and cry1cry2 were used as the plant materials and analyzed the regulation of Auxin biosynthesis and Auxin polar transport by blue light receptor in Arabidopsis . (2) A large number of transfer GFP: cDNA randomly transgenic Arabidopsis were used as the plant materials ,through the fluorescence microscope to observing and screening the expressing GFP: cDNA gene, and clone the cDNA that expressed in cell nucleus, then study the structure and function of this gene further more. The result is as follows:1 A Preliminary study on regulation of Auxin biosynthesis and Auxin polar transport by blue light in Arabidopsis.1.1 Study on the inducement of hypocotyl elongation by IAA.The auxin biosynthesis or the auxin polar transportation enhanced under the continuous blue light in blue light receptors mutant materials, because of IAA saturate in Arabidopsis .if use outdogenesis IAA, the hypocty1 elongation will be inhibited because IAA is excessive; Or IAA content itself has already satisfied the demand, make the superfluity of IAA and reach the best state, when use IAA outside, plant have no change, and inhibited the hypoctyl elongation in contrast.1.2 Analysis the inhibition of polarity transportation (NPA ) to hypoctyl elongation. Result indicated that with NPA density increaseing, NPA will inhibit hypoctylelongation further under the continuous white light, blue light and red light but blue light . The inhibition of polarity transportation (NPA ) to hypoctyl elongation with blue light will be smaller than continuous white light and red light in blue light receptors mutant. So the blue light enhanced the expression of key gene in auxin polar transportation.1.3 Analysis the regulation of the key gene of auxin biosynthesis and axuxin polar transportation by blue light with different time and differentlight intensity .Treated with continnous blue light, the expression of IAA5 increased during the 4-8 h, the expression of IAA5 is higer in cry1-304 and cry1cry2 than cry2,while the wild type drop. Indicated that blue light receptor participate in IAA signal pass way. That is to say the blue light receptor is a negative factor to regulation the expressing of IAA1 and IAA5 . Treated with higer intensity of light. (40Imol m-2 s-1 ), the expressing of IAA1 and the difference is more obvious.Dealing with different time and different intensity of light, AtAPM1 and AtAPPl witch participate in auxin polar transportation and expression of wild type and mutant mode nearly at all the same, there is no change. Indicate two gene have no relationship with the hypocotyl elongation .The results show that blue light receptor mutant long hypocotyl caused by the fact that the signal of IAA conducts and strengthens,and have no relationship with the auxin polar transportation and auxin biosynthesis.2. Analysis the localization and function of GFP:: cDNA that insert in the...