Branch Of Peptide Synthesis And Protein Chip Production

The author synthesized the multiple antigenic peptides with solid phase method. The MAPs were made of the human cytomlegalovirus's antigen epitope. And the DIGFA method was applied to detect the antibody against HCMV. The result shows:The MAPs were constructed by two kinds of scaffolding: linear lysine-tree scaffolding and branchy lysine-tree scaffolding.One of the synthetic methods of the MAP is to connect subsequent amino acid one by one with the lysine belonging to the lysine-tree scaffolding. Because of the steric hinderance effect, synthesis becomes more and more difficult. And for so many crippled peptides in the MAP, the reactivity of it is not so good.The MAP connecting pured peptide to the lysine-tree scaffolding has less crippled peptides and better reactivity.The MAPs linked to different lysine-tree scaffolding have different immunogenicity. The reactivity of the MAP made by linear lysine-tree scaffolding is better than the reactivity of the MAP made by branchy lysine-tree scaffolding.The protein chip made by coating the MAP on the nitrocellulose membrane could be developed to be a new rapid clinical detecting method because of its high binding activity and the antigenic activity of the HCMV.