Baculovirus (baculovirus) Membrane Fusion Protein

There are 3 chapters in this thesis: Chapter one is a general introduction of two kinds of baculoviral envelope fusion proteins(EFP), GP64 and F proteins. The structure and functions of these proteins and the application of GP64 for surface display as well as the substitution of several viral EFPs were summarized. In chapter two, for research of the relatedness of EFPs of different group NPV, two recombinant baculoviruses were constructed via Bac-to-Bac system. HaSNPVgp64+egfp+ possessing GP64 of AcMNPV and EGFP, and HaSNPVegfp+ with an EGFP as control. Western blot analysis indicated that GP64 was expressed in HzAMI cells infected with HaSNPVgp64+egfp+ and was packaged into progeny BVs. Plaque experiment indicated that HaSNPVgp64+egfp+ can produce infectious virions in Sf21 cells. In chapter three, the relatedness of GP64 and F protein was studied by substitution experiments. On the basis of Bacmid HaBacHa133-, AcMNPV gp64 was inserted into its polyhedron locus by transposition, the recombinant Bacmid was named HaBacHa133-gp64+egfp+. Two methods were used to detect whether the replacement could rescue the deletion of HA133 in HaSNPV-HzAMI system. First, Sf9 and HzAMI cells were infected with successfully transfected supernatant.Second a transfection-plaque method was used. With both methods, the production of infectious progeny virions was not observed. The results indicated that GP64 would not substitute for the function of HA133.