Cloning And Sequence Analysis Of Three Kinds Of Mosquitoes Co ¢ò Gene

Mosquitoes (Diptera: Culicidae), by nature of its association with problems of human and animal health, are medically important insects. Anopheles sinensis^ Aedes albopictus and Culex quinquefasciatus are all the most common mosquitoes in China. In this article, the cytochrome c oxidase subunit II gene (COII) in the mitochondrial DNA of the three mosquitoes was amplified by the polymerase chain reaction (PCR) technique, then was cloned into the pUCIS T-vector. The complete sequences of the three COII genes from Anopheles sinensis^ Aedes albopictus and Culex quinquefasciatus were sequenced and analyzed. The homology of the COII gene nucleotide and the deduced amino acid sequences of COII among three mosquitoes varied from 84.1% to 87.9% and from 85.1% to 89.5%, respectively. The C+G content of COII gene is about 23.2%~24.9%, and the frequency of transversion is higher than that of transition. The three mosquitoes were compared with others according to the COII gene, molecular phylogenetic tree was constructed base on the sequences of COII gene. The evolution history and relationships among those mosquitoes were discussed. Aedes albopictus and Culex quinquefasciatus are closely related species for their COII molecular level according to COII gene. The COII gene shows promise for resolving evolution and systematics of mosquitoes.