Several Freshwater Gastropods Molecular Genetic Classification

From April 1998 to April 2001 ,by means of Molecular genetics, including foot protein esterase enzyme and random amplified polymorphism DNA (RAPD) in six species of fishwater snails based on morphologic classi1', The Arocinma longicorrns. Parafossarulu st riatzdus Parafossanduseximius Stenothyra glabra SemLstdcospira cancellata and Bellamyapuriflcata Seiniszdcospiraglabra and Bellamya punftcata Semfrulcospira glabra and Bellanwapw7ficata have been studied in detail respectively which belong to Mesogaslropoda. According to the result; the classical taxonomy on Hydmbiidae is not capable of reflection the relationships of Gaslropod phylogeny , the results of the expenments are coincided th recent modified taxonomical system in Mesogastropoda abroailThe bands of foot protein with eleclrophoresis are remaikably different in the different families of the Gastropod, but identical in the same species. The variation of the foot protein in different species of snails demonslrates the corelationships of the Gastmpodphylogeny. The most important feature for distinguish snails by foot protein elecUophoirarn are quantities, relative migration (Rin) and scanarea of the main bands in electmpborgram. The Amcinma longicornfr Parafossandu striatzduc andParafassandu aimius has single main band which in 0.16 Rni data the scan area from 45 mm2 to 612011102mm .TheStenothyragVabraare3mainbandsin0.08.and0.l7Rxnwith20and22nim.SemLzdcospira cancellata and Bellamyapurzftcata are in 1 and 2 main bands in 0.07 and 0.06~ 0.17Rni with 35 and 16. 22 mm2 respectively. There are a few differences in subordinate bends amongclose species of snails.From the result of three gene loci of esterase isoenzyme, the pattern of esterase maps are different25on the zymogram in different species. There are obvious genetic polymorphisms among populations of each species. The distribution of esterase marks are remarkably distinction in different distance coefficients of esterase are from 0.218 to 0258 among Alocinma longicomis Parafossandus striatulus and Parafassandus eximiu and the distribution of esterase are homologyThe data of Stenothyra glabra SemLsulcospira cancellata and Bellamyapunflcata are from 0.566 to 0.608 which distributions of esterase are much different than that of former. Therefore the Stenothyra glabra is farness in relations with other snails else.Using four lObp random primers, total 64 bands were produced fi~rn 6 species of snails. TheAlocinma longicornis Parafossandus striatulus andParafossandus eximius have close relationship which fragments shared (F) are from 0.703 1 to 0.7343 and the genetic distance(D) are between 0.2422-02968 among them. The fragments shared (F) and the genetic distance(D) are0.3437-0.4687 and 1.0848-1.4648 to former among Stenothyra glabra Semiszdcospira cancellata and Bellamya purzficata ,are evaluated by cluster analysis to drawing a phylogeny showing the relationship among species, the relations of Alocinma longicornis Parafossarulus siriatulus and Parafossandus eximius belong to the difference of Species or Genus, compared Stenot/yraglabra Semis'ulcospira cancellata and Bellamyapurzflcata with above species ,the latter belong to the difference of Families. From results of the experiments, we suggest that those RAPD marks would be constmcted steady molecular genetic maps pool for freshwater Gastropod.