Biological Effects Of Low Energy Ions Of Physiological And Biochemical Characteristics Of A Number Of Studies

First, the development and application of radiation梑iologics and bio梕ffect of low梕nergy ion beams were reviewed, and the compositionworking condition of the low energy ion set were summarized. Second, the implanted bio梕ffect of bio梞olecular~ the micro analysis of implanted seed surface and the change of physiology~ biochemistry of implanted plant were researched.The special 搒addle type?of relation between livability and the dose of implanted ion is still an interesting problem. In the chapter 2, the structure damage of Thymine and the relation between the damage and dosewere researched by Ultraviolet spectra ~ Infrared spectra and XPS. Respectively the fact that the relations between ultraviolet livability and the dose for both~and Ar~ are 搒addle model?shows that the mass deposit is not the main factor of 搒addle model?. It can be showed from Infrared spectra that the change of typical peak position can retrieve with the increase of dose, and the change of the relative strength of these typical peak has the tendency to decrease~ increase and decrease again. Also it can be proved that the structure is damaged~ restored and damaged with ion beams implanting, and the restoration has some tendency, not accidentally totally. The result of XPS of Thymine by 2 X lO?N~/cm2 implanting showed that the relative content of C~ N~. 0 changed obviously and the chemical environment of these elements changed at the same time. The damage of small bio梞olecular by implanting reflects the initial damage of low energy ion beam implanting. This has important reference value to research the implanted effect of biologic big molecular and the whole living body.In the third chapter, the micro梥tructure analysis of implanted surface cell of soybean was done. The sputtering effect produced by ion beamsIvimplanting was researched. Moreover with adding the dose, the damage became more serious.In the forth chapter, the physiology of soybean to resist the cold harm and the influence of membrane?s penetrating produced by ion beams implanting was researched. The experiment was carried out from three aspects of the seep content of inorganic ion, the seep of solubility sugar, the seep of acid matter. Under the condition of cold harm, the result that the seep content of the three kinds of matters in low dose had a increase tendency compared to that of the sample only in low temperature but riot being implanted was found. This showed that the implanting of low dose made the membrane damaged and made the penetrating added. The higher dose implanting made the seep of these matter decreased, too. The charge exchange effect could change the membrane抯 electric potential. Moreover the energy transform and matter transport could be influenced. This could make the physiology function reach an excited state, and not only restrain the harmful influence produced by ion beam implanting, but also restore the damage of physiology in low temperature.In the fifth chapter, the influence of low energy ion beams to soybean抯 estarase isozyme and wheat抯 peroxidase isozyme was studied using poiy acrylamide gelatum electrophoresis. After repeating the experiments several times, the following results were found. The activity of soybean抯 estarase isozyme had the tendency to increase and decrease with adding the implanting dose. But the vacuum contrast sample抯 result showed that the activity of estarase had the tendency of increase with prolonging the time of vacuum. This show the increase and decrease tendency of implanted sample was produced by ion beam implanting, not by vacuum. The results of wheat抯 peroxidase isozyme, including determining the activity, the result of PAGE, the result of thin layer scan, show that the process of isozyme activity can be changed . And with adding the implanting dose, the time of startV4living body抯 protect system was postponed. When the implanting dose was not very high, the activity of peroxidase isozyme can be increased. This will pro...