The Effect Of Membrane Rafts On Lateral Mobility Of Gaba_b Receptors

Gamma-aminobutyric acid (GABA) receptors are the G-protein-coupled receptors for GABA, the main inhibitory neurotransmitter in the mammalian central nervous system. GABA_B receptors are unusual in that the homologous GABA_B1 and GABA_B2 subunits need to heterodimerise in order to reach the cell surface.Lipid rafts are specialised microenvironments within biological membranes that are enriched in cholesterol and sphingolipids. Lipid raft domains are platforms for plasma membrane signalling complexes. They are key regulators of G protein coupled receptor function. The G protein coupledγ-aminobutyric acid receptor B (GABA_B receptor) co-isolates with lipid raft domains from rat brain cerebellum. So in this paper,we investigated that the regulation of rafts for lateral diffusion of GABA_B receptor by Totle internal reflection fluorescence microscopy in transfected HEK293 cells.So in this paper,we investigated that the regulation of rafts for lateral mobility of GABA_B receptor by Total internal reflection fluorescence microscopy in transfected HEK293 cells.We discovered that GABA_B2 receptor regulated the translocate mobility of GABA_B1 receptor,while the mobility of GABA_B1 mutant, which was able to atomatic ally to the cell membrane with a radom trajectory. Was not regulated by GABA_B2 according to sigle-molecule imaging. Lipid raft domains are platforms for plasma membrane signalling complexes. We found that the diffusion coefficient of GABA_B receptor heterodimers increase when cholesterol is distrupted,and the lateral mobility of GABA_B receptor is regulated by lipid raft. Compared to GABA_B1a receptor, GABA_B1b receptor is easier to translocate lipid to the lipid raft.When the cells were treated with MCD,we found that Median Instantaneous Diffusion Coefficient of GABA_B1b-containing heterodimers is higher than that of GABA_B1a -containing receptor.This paper only a first investigate for lateral mobility of GABA_B receptor and are underpinned for researching of molecular mechanism.