The Experession Map Of The Cell Membrane Related Proteins Of Acidiphilium Dx1-1

With the reseach of iron respiration, Acidiphilium sp.has been paid more attention to,because of its iron respiration,. In the application of bioleachingAcidiphilium sp. plays an important role during form ation of precipitated ores, which can cooperate with other microorganisms in bioleaching process.Via reducing the poisonous impact of organics to bioleaching orgnisms providing low pH enviroment, Acidiphilium sp.can effectively increase the efficiency of bioleaching.Nowadays,a microbial fuel cell has been developed using the acidophile, Acidiphilium cryptum, as the anode biocatalyst。In this study, the cell membrane related proteins of Acidiphilium DX1-1. were extracted and separated by SDS-PAGE. And then,subjected in-gel digestion.The peptides,which were seperated by liquid chromatography,were directly subjected for online analysis by the coupled tandem mass spectrometry. With Mascot search engine,262 identification proteins were identified.The protein export signal, subcellular location and function of each identified protein were predicted. Among these identified proteins, 13.7% possess export of the Sec type,27.86% show a twin arginine translocation (Tat) signal,18.32% have lipoprotein signal peptides.14.9% have a C-terminal GPI-anchor signal sequence,and 2.67% have both C-and N-terminal signal sequences. According to satistics assay, the molecular weights of these proteins are distributed in the range of 4KDa to 120KDa;40% of which show the pI values below 7,60% higher than 7, and 27.48% over 10.30 identified proteins in this study are coshared between cell wall and cellmembrane enrichments, which include 3 energy metabolism related proteins,8 transport and binding proteins,2 Protein synthezing related proteins,2 modification related proteins,8 Cell envelope related proteins, and 4 proteins with unknown function.The Acidiphilium DX1-1 membrane proteomics in this study will provide solide foundation in clarifying the mechanisms of iron respiration of Acidiphilium DX1-1 under different enviroments.