Study On Extraction, Purification And Function & Activity Of Rapeseed Phospholipids

Phospholipids are an important component of the biomembrane. It is a sort of the natural enhancer of the high-nutrition. And it also is a kind of natural surfactant. It is widely used in leather, animal feed, textiles, cosmetics and other fields, with high scientific research value and economic value. This experimental research maked rapeseed oil residue as raw materials, and surround a central issues, including the phospholipids extraction, purification, quality accreditation, activity. It maked theby-products of representative crops （rapeseed） inhubei Province--rapeseed oil residueas object of research, phospholipids were separated and extracted through the acetone and ethyl ether extraction methods. And then phospholipids were bleached by H₂O₂ and punficatied by means of column chromatography. Technology of Ultraviolet and infrared scanning did an analysis of product quality, and the activity of the product was studied that mice were determined the protective effects of oxidative damage of heart and liver organizations by incepting phospholipids, detection of mice SOD activity and LPO content.Through this research, the main conclusions are as follows:1. The rapeseed oil residue were measured moisture content, acid value, peroxide value, saponification value, unsaponifiable matter , the total content of fatty acids and phospholipids. Such as the total target, the total phospholipid content was 28.89%.2. Using the method of acetone extraction, the optimum conditions for the parameters of concentration rapeseed phospholipid obtained were that the amount of acetone was 12mL/g, the extraction time was 41 minutes and the extraction temperature was 48℃.3.Making H₂O₂ as discolored reagent, the best conditions of concentration rapeseed phospholipids decolorization were bleached temperature of 30℃,H₂O₂ concentration of 5.85%, bleached time of 14.5minutes. Decoloration phospholipids weregolden brown paste.4.Respectively, using neutral alumina and silicagel as filler materials, the research of purification was maked for detreated phospholipids, The rates were 66.62% and 51.93%.Various aspects data shows that the effects of purification by neutral alumina was better than silicagel. Purified phospholipids and sigma standard lecithin for the ultraviolet and infrared figures of the comparative analysis showed that quality of purification phospholipids was veryhigh and the structure was basic agreement.5.The function & activity of phospholipase, the protective effects of peroxidative damage for the mouseheart, liver and other organizations by incepting the phospholipid productshad been studied.The experimentation detectived the effects of the phospholipids of mice superoxide dismutase （SOD） activity and lipid peroxide （LPO） content. The experimental results showed that the protective effects of peroxidative damage was very obvious, and it also showed some the dose effect, the effect of 200mg/mL was most obviou. The maximum enhanceed rate of SOD activity and the maximum decreased rate of LPO content can reach 65.6% and 45.4% inheart, 53.0% and 22.0% in liver.