Studies On The Liquidfermentatiionand Anti-Oxidant Effect Of Ganodoerma Sinense

With the development of modern society, the material become richer and richer. But the problem of human health is faced with acid test at the same time. Among the problem, the oxidation is always paid attention to. More and more research show that oxidation is an important step of aging. Free radical and oxidizing agent resolve the cell nucleus organization and affect the metabolic function, even to cause different health issue. The passive smoking, excessive drinking, radiation and environmental pollution can produce free radical.Some research indicates that the antioxidant system can be matched with the immune system, which is a system with perfect and complicated function. More powerful the antioxidant of the body was, more healthy the body was.Ganodoerma Sinense was chosen as object of research. A lot of fermentation products used for industrial productions were obtained by method of liquid fermentation. Edible funguses are safe and contain a variety of biological active component. In order to explore the fermented product antioxidant activity of Ganodoerma Sinense, the antioxidant ability in vitro was studied. The main conclusions were as follows:.(1) The experiment discussed the method of liquid fermentation to obtain mycelium of Ganodoerma Sinense. By method of single factor and orthogonal designs, the liquid spawn medium formula and culture conditions of Ganodoerma Sinense in submerged culture with shake flask were optimized according to the index of mycelia biomass. The results showed that the best carbon source and nitrogen source are glucose and peptone respectively. Consequently, the optimal culture medium component was:30g/L glucose,3g/L peptone,1.2g/L KH2PO4,0.8g/L MgSO4,0.01g/L VB1. Furthermore, the optimum culture conditions were as follows: liquid medium volume24%(v/v), inoculation quantity15%(v/v), culture temperature25℃, shaking frequency140r/min, initial pH,7days incubation time. Based on shake flask fermentation, Ganodoerma Sinense was fermented in airlift fermenting tank which contained5L submerged culture.The air-flow rate was0.2Nm3/h.(2) Ganodoerma Sinense fermentation functional products contained polysaccharide,protein,flavones, polyphenol. The yield of extracellular polysaccharide was0.146mg/mL and0.104mg/mL; flavone0.0076mg/mL and0.011mg/mL; polyphenol0.0348mg/mL and0.0417mg/mL respectively.(3) Ganodoerma Sinense fermentation products’ antioxidant research include reducing power, capability of scavenging-DPPH,·OH and·O2-. The antioxidant experimental results showed that Ganodoerma Sinense fermentation liquid had good antioxidant activity. Especially, scavenging rate of·OH reached up to80.45%.