Preparation And Application Of Malachite Green And Bisphenol A Molecular Imprint Polymer Fibers

Molecular imprinting technology is used a certain target molecles as template to prepare molecularimprint polymer which has the advantages of specific selectivity and identification to the templatemolecules and the compounds with similar structure. It has been widely applied in the area of food safety,environment detection and analysis, drug testing and analysis, sensor and chiral resolution. Two kinds ofmolecular imprint polymer solid phase microextration fibers were prepared and applied in the foodannalysis which included fish feed and tap water.1.A novel detection method was established by combing the molecular imprint solid phasemicroextration technology with HPLC. Malachite green (MG) molecularly imprinted polymer (MIP) wasprepared within the capillary with530μm inner diameters. It was carried out by microwave irradiationusing malachite green as a template molecule, α-Methacrylic acid (MAA) as a functional monomer,acetonitrile (ACN) as a porogenic solvent, ethylene dimethacrylate (EDMA) as a crosslinker,azodiisobutyronitrile (AIBN) as a thermal initiator. The resulted MIP fibers were pushed out from thecapillary, eluted and inserted in the capillary again, which successfully used for the SPME procedure. Thefactors affecting the extraction of MG, such as the molar ratios of template/monomer (MG/MAA),concentration of NaCl, extraction and desorption time, and extraction and desorption solvents wereinvestigated in detail. The selectivity of the MIP fibers was compared using MG analogues crystal violet(CV) and non-analogue SudanⅡ. It was also employed for solid phase micro-extraction (SPME) of tracemalachite green in the fish feed followed by high-performance liquid chromatography (HPLC) detection.Under the optimal conditions, the linear range of MG was10-600μg/L, the detection limit (LOD) was1.23μg/Land the recovery of spiked fish feed sample was88.7～113.9%.2. The fast preparation of molecularly imprinted polymer fibers (MIP) was reported using bisphenol A(BPA) as a template molecular, acetonitrile (ACN) as a porogenic solvent, α-Methacrylic acid (MAA) as afunctional monomer, ethylene dimethacrylate (EDMA) as a crosslinker, and azodiisobutyronitrile (AIBN)as a thermal initiator. It was carried out within a capillary of530μm inner diameter (I.D) by microwaveirradiation in7min. The resulted BPA-MIP were pushed out from the capillary, eluted in a vial and insertedin the capillary again followed by the application for the solid phase microextraction (SPME) procedure. Their extraction performance was investigated in detail by varying the molar ratios between template andmonomer (BPA/MAA), concentration of NaCl, extraction and desorption time, pH value and desorptionsolvents. The selectivity of the prepared MIP and NIP fibers was comparatively evaluated by selecting twostructurally-related compounds including phenol (P) and4-phenylphenol (PP) and non-analoguedicyandiamide (DCD). The established method was successfully applied for the pretreatment anddetermination of BPA from beverage sample coupled with HPLC. Under the optimal condition, the linearrange of BPA was10~400μg/L, the detection limit (LOD) was0.45μg/L and the recovery was88.4~102.8%for mineral water. The results demonstrated that the developed method could determine BPAin the tap water with some advantages of simple pretreatment, rapid analysis, low limit of detection and lowconsumption with organic solvent.