Preparation And Research Of Novel Multiple Particles For Chromatography

The major research of chromatographic separation of particles are focusing on two points:The physical structure and chemical modification. In this work, sepharose gel chromatography particle as the substrates, we talk about the influence of the adsorption performance of core-shell particles which with different porosity and modification density distribution, and the preparation of particles with hydrophobic and hydrophilic group simultaneously modification.Firstly, for the different porosity and modification distribution particles, we start with a simulation to clearly show the column efficiency affect by different structure in the core and shell. The results indicated that the effect by changing porosity and modification in the shell is more important. Then we perparaed the particles with different porosity and modification density distribution. From the result of BSA static and kinetics adsorption, it shows that core-shell particles with macropores shell accelerated the protein mass transfer in the particle, and the core without macropores would increase the adsorption capacity. Partices with high modification density and macropores in the shell, a non-macropores and low modification density in the core show a good Performance to separation protein(particle3-2). Then, compared with agarose particles, column packing with particle3-2show a higher efficiency, a well pressure capacity, high plate numbers and low tailing factor.Finally, we prepared a novel chromatography particle have multiple adsorption structures (hydrophobic and ion-exchange structures). In this work, DEAE as the ion-exchange group and phenyl as the hydrophobic modification. The particle is made of agarose gel. Particles with different modify density were prepared by differrent orders. The results show that the modification density of first group is larger than the second modification. Moreover, particle adsorption is similar with the adsorption of high modify density strucutre, but particle adsrption is influenced by the other modification group. For particle with high phenyl modify density, the adsorption of these particles were influenced by two modified structures, the adsorption capacity of multiple modified structure is higher than only one modified structure in phosphate buffer (pH=7.0). Furthermore, the fitting results of static adsorption show that multiple modified structure have double adsorb sits.In this work we analyzed two kinds of particles, and discussed the influence of column structure and modify density. The mechanism of multiple modified structure were studied. This work can provid basic reach information for the future work.