Intelligent Control For Preventing Bacterial Contamination Of Bio-Ethanol Ferementation By Adding Bacteriophage

The depletion of fossil resources exploits quickly, excessive use not only cause environmental pollution, but also bring serious impact to our daily lives. In recent years, air quality has got to non-compliance in more and more cities. The source of phenomenon is undue consumption of traditional energy sources, the main power supply of motor vehicles is still gasoline diesel in China. Electricity and heating rely mainly on coal, these produce the pollution of CO, hydrocarbon and Pb et al, which lead mass of harmful substances into the atmosphere directly, the haze weather has bad effect on our health. The development of clean, sustainable and renewable power sources is imminent. Gasoline has accretion ethanol that can not only improve the gasoline octane number and oxygen content, but also make it burn more richly. The addition of ethanol can help reduce the amount of CO, hydrocarbons in automobile exhaust. In many other countries and our country promote the application of ethanol gasoline. Industrial ethanol production methods include fermentation and synthesis. Saccharomyces cerevisiae is the main species alcoholic fermentation. Instrument leakage, seed pathogen and other reasons can result in bacteria fermentation in the process of fermentation producing ethanol. It is a difficult problem to solve in industry. Because the loss of bacteria pollution is quite amazing in industrial fermentation, we must seriously handle this problem. For bacteria pollution, people have fumbled a series of methods, containing addition antibiotics, antiseptics and lactate from the long-term production practice. The first method can cause drug residue, the second way can’t recycle and waste more sources.For the sake of solving this problem, we came up with a new way with using bacteriophage to arrest the growth of lactic acid bacteria. Using bacteriophage restrained bacteria problem in the fermentation. We checked out the number of yeast, bacteria, pH, reducing sugar, the final stage ethanol content of fermentation, and used the software PASW STATISTICS correlation analysis, establish regression model, and found out the fermentation process Bacterial contamination of the most relevant environmental factors. We based on the idea and made use of wrapping material wrapped in preparation for bacteriophage particles and the formation of the most relevant environmental factors response released. First, preparing chitosan microspheres used scanning electron microscope (SEM) to measure its shape and size. Microspheres were ground by liquid nitrogen, and observed the internal structure of the microspheres by transmission electron microscopy (TEM), microspheres prepared to prove whether a core-shell structure, whether they had the basic conditions for the package material. Microsphere had inclusions of bacteriophage, and wrapped microspheres were swelled at different pH conditions, the ultrastructure of microspheres were measured to verify the success of chitosan microsphere inclusions bacteriophage particles, and could be wrapped at the different pH response. The microspheres wrapped bacteriophage particles, which were added to the simulated contamination of fermentation system. We compared with the simulated contamination of fermentation system without wrapped microspheres by measuring the final periods of fermentation ethanol content. Experiments showed that the final ethanol content of the microspheres wrapped bacteriophage particle were obviously higher than the simulated contamination system (P<0.01). It indicated that contamination could be effectively controlled fermentation system by adding microspheres wrapped bacteriophage particles.