Synthesis,Characterization And Application Of Mercury Ion Near-Infrared Fluorescent Probe

Heavy metals mercury ion is widespread concerned by people as long-term environmental pollutant. Environmental protection and food safety to human health and social stability is important. Therefore, heavy metal mercury ion in the environment detection has been one of the research focus. Fluorescent detection method is simple, low-cost, specific, sensitive and can be visualized in situ detection. The method is very challenging for the rapid detection of heavy metal ions.Based on this, this work designed and synthesized a fat-soluble mercury ion near-infrared fluorescent probe based on mercury hydration reaction of alkyne for the first time, which took heptamethine cyanine fluorescent dye IR780to be a fluorescent signal unit, propargylamine for identifying ligands, respectively. By. methods of thin layer chromatography, mass spectrometry, H NMR, the synthesized fluorescent probe was characterized. The results of selective experiments showed that the probe had good selectivity for mercury ion. At pH=6.2, the linear range was0-300ppb, and the limit of detection was20ppb, and the ratio of mercury ion to the probe was1:1. Satisfactory recoveries were obtained and ranged from98.4%to103.7%at three spiked levels in real tap water samples with high precision, which indicated that the established method of fluorescent probe was successfully applied to the quantification of mercury ion.A water-soluble heptamethine fluorescent dye was synthesized for the good photostability and water-solubility and the nucleophilic reaction for the central chlorine atom. The sulfonic acid group, the benzyl group and six bridge structure were introduced at the two3H-indo parent rings of the heptamethine cyanine dye. By methods of thin layer chromatography, mass spectrometry, H NMR, the synthesized fluorescent probe was characterized.Furthermore, two three-legged pyridine ligands-aminomethyl-(2-pyridylmethyl) amine (L1) and6,6’-diamine methyl-(2-pyridylmethyl) amine (L2) were sysnthesized and were charactervized by the methods of thin layer chromatography, mass spectrometry, HNMR.