Study On Extraction，Separation And Stabilization Of Blueberry Skin Anthocyanins

Blueberry is Ericaceae Vaccinium genus，our country have a rich blueberryresources．The research shows that blueberry anthocyanins substances exist in peeland anthocyanins substances have a wide range of physiological functions andmedicinal function such as eliminate eye fatigue，protect eyesight，anti-oxidation，anti-aging，anti-ulcer，anti-inflammatory，anti-cancer and so on．In this paper，the blueberry frozen fruit peel as a raw material， it systematically studiedanthocyanins extraction and purification process and stable performance，it is veryimportant to development and use the blueberry resources．This paper systematically anthocyanin extraction process；macro porous resinpurification methods； compare silica gel thin layer chromatography and highperformance liquid chromatography separation of anthocyanins， liquidchromatography-mass spectrometry to conduct a preliminary analysis ofanthocyanin component；study the stability of blueberry skin anthocyanins．Themain conclusions are as follows：1．The optimal conditions of extraction blueberry skin anthocyanins were asfollows：volume fraction of40％acidic ethanol（pH value2.0），extraction time60min，liquid ratio10mg:10mL，extraction temperature50℃，the anthocyanincontent of7.5660mg/g peel．2．The conditions of static absorption and desorption by AB-8macro porousresin were that adsorption equilibrium time was4h；desorption equilibrium timewas1h，anthocyanin solution pH was2.0；macroporous resin to its adsorptioncapacity relatively strong；volume fraction of40％ethanol solution（pH2.0）as theeluent．The conditions of dynamic absorption and desorption were that sample flowrate and desorption flow rate were1.0mL/min，sample volume of220mL，150mLvolume fraction of40％acidic ethanol as eluent．After AB-8macro porous resinpurified，the color price of blueberry skin anthocyanins was52.10．Silica-gel plateas chromatography media，water-ethanol-n-butanol-ethyl acetate (11:9:25:36) andn-butanol-acetic acid-water (4:1:5) as developing solvent separation effect arebetter． Blueberry skins was found to contain12anthocyanins by liquidchromatography-mass spectrometry．3．The blueberry skin anthocyanin was stable below60℃，and more sensitiveto light；anthocyanin more stable under the conditions of the pH value of less than 5.0；the oxidation and reduction resistance of the anthocyanins were relativelypoor；glucose has hyperchromic effect；potassium sorbate protect the color andincrease stability of anthocyanin； Na+has hyperchromic role， and the highconcentration of Na+to increase the stability of anthocyanins；Ca2+，Mg2+，Zn2+hashyperchromic role，and nor significantly effect on color glycosides stability；Al3+hashyperchromic role， and low concentrations of Al3+on the stability ofanthocyanins had no significant effect；K+cannot increase the absorbance value andno significant impact on the stability of the anthocyanin；Cu2+，Fe3+no obvioushyperchromic role，and have undermine the role to anthocyanins．...