Oxidation Of Adrenaline Catalyzed By Octacarboxylic Phthalocyanine Iron And Its Supported Phthalocyanine And Their Applications

Adrenaline is one of the catecholamines, as a neurotransmitter it’s secreted byadrenal medulla and some pheochromocytes in sympathetic neurons, and it plays avital physiological role in human body. Because of the low level of adrenaline inbiological environments, its detection has large interferes and weak signals, theexisting detection methods can not fully meet its requirements for high detectionsensitivity, precision and anti-disturbance capability. Therefore exploring a rapid,sensitive and simple detection method is very significative, and we still have urgentneeds to explore new methods for adrenaline detection.Metal phthalocyanine has excellent catalytic performance as biomimetic catalyst,it also has very stable chemical properties and its preparation is simple. However, thecatalytic performance of metal phthalocyanine is greatly limited by its poordispersibility in the aqueous solution.In response to problems mentioned above, the contents of this thesis are in thefollowing two aspects:(1). A water-soluble metal phthalocyanine—OCFePc has beensynthesized, and a carrier phthalocyanine which are loaded on porous silica—OCFePc-SiO2has been prepared. These two metal phthalocyanines are used asbiomimetic catalysts in the catalytic oxidation of adrenaline respectively, and somefactors which affect the reaction have been studied.(2). With the two catalyticoxidation system a fluorimetric determination based on fluorescence quenching ofCdTe quantum dots and a fiber-optic oxygen sensor for the detection of adrenalinehave been studied.OCFePc is synthesized with the phthalic anhydride and urea method, and we getOCFePc-SiO2by covalent cross-linking OCFePc on silica carrier. These methods aresimple and the product is easy to purify. OCFePc-SiO2has large specific surface area,most OCFePc are linked to SiO2in the form of single molecule, and that leads to itsgood dispersity in the catalytic system. In addition, OCFePc-SiO2has excellentreusebility.With the aid of oxidizing agent BuOOH, adrenaline can be quickly oxidizedthrough the catalysis of OCFePc and formed adrenochrome. The rate of the catalytic oxidation will rise with the increase of catalyst concentration, BuOOH amount andtemperature, the optimum solution pH is8.0. With the catalysis of OCFePc-SiO2,adrenaline can be oxidized to adrenalin-o-quinone just using O2as oxidant. Amountof catalyst, temperature and pH have the same influence on the catalysis ofOCFePc-SiO2as they have on OCFePc.Both the fluorimetric determination of adrenaline based on fluorescencequenching of CdTe quantum dots and the fiber-optic oxygen sensors for adrenalinecan achieve rapid response. Their liner ranges are0.2-20ug/mL and0.05-20ug/mL,and detection limits are0.2ug/mL and0.05ug/mL respectively. Compared tofluorimetric determination, the detection of fiber-optic adrenaline sensor has a widerdetection range, a lower detection limit and better anti-disturbance capability.