Synthesis And Study Of Fluorescent Probe’s Properties Characterization Based On Rhodamine B And PAN

The recognition and sensing of the biologically and environmentally important metal ions has emerged as a signicant goal in the field of chemical sensors in recent years. Among the various analytical methods, fluorescence has been a powerful tool due to its simplicity, high detection limit. Rhodamine dyes have high fluorescence quantum yield, large absorption coefficient and excellent photophysical properties. Therefore, rhodamine based material have become one of the perfect chromophores for making fluorescent probes.In this paper, seven rhodamine B derivatives and three PAN-based derivatives were designed and synthesized, including nine new products. These compounds structures were confirmed by IR、HRMS、1H NMR and13C NMR.A novel rhodamine-based fluorescent probe L1and L2with two "S" groups was characterized. As expected, L1and L2exhibited high selectivity and sensitivity for Hg2+over other commonly coexistent metal ions. Its selectivity is excellent, and the detection limit was measured to be1ppm. Furthermore, fluorescence imaging experiments of Hg2+ions in living EC-109cells demonstrated its value of practical applications in biological systems. A novel colorimetric and off-on fluorescent chemosensor L3and L4was designed and synthesized, which showed reversible and highly selective and sensitive recognition toward Cr3+over other examined metal ions in aqueous solution. Chemosensor L3and L4exhibited a stable response for Cr3+with a detection limit of1ppm. Furthermore, fluorescence imaging experiments of Cr3+ions in living EC-109cells demonstrated its value of practical applications in biological systems.A novel colorimetric and off-on fluorescent chemosensor L5, L6and L7was designed and synthesized, which showed reversible and highly selective and sensitive recognition toward Fe3+over other examined metal ions in aqueous solution.Three novel colorimetric fluorescent chemosensor L8, L9and L10were designed and synthesized, which showed reversible and highly selective and sensitive recognition toward Cu2+over other examined metal ions in aqueous solution. Upon addition of Cu2+, the solution of chemosensor L8resulted in a color change from color-less to obvious purple color. Then to this solution, the GSSG was added and the solution resulted in light colored. These significant changes in color could be used for naked-eye detection. Furthermore, fluorescence imaging experiments of Cu2+ions in living EC-109cells demonstrated its value of practical applications in biological systems.