Simultaneous Determination And Application Research Of Industrial Dyes In Food

Foods are the necessities of everyday life，and the state and society have alwaysattached great importance to food safety problems. Because many industry dyes havemany side effects such as carcinogenesis teratogenesis and mutagenesis, they areforbidden using in food processing. Many international organizations have severerestricts about synthetic dyes. In this study, liquid chromatography–tandem massspectrometry (HPLC-MS/MS) was applied to analysis Chrisoidine, Auramine O,Rhodamine B, Para Red and Sudan I~IV dyes. HPLC-MS/MS was a good detectorfor quantitative and quantitative analysis of food samples.1. A LC-MS/MS method for simultaneous determination of eight industry dyesin chilli products was proposed.According to the physical and chemical properties of Chrisoidine, Auramine O,Rhodamine B, Para Red and Sudan I~IV, after several experiment to explore, thefinal quantitative method are as follows:The chili products were extracted with acetonitrile-water(v:v=9:1) and assistedwith ultrasound. The target analytes were separated on a C18column with gradientelution using5mmol/L ammonium acetate solution containing0.1%formic acid andmethanol as mobile phase, and then detected by MS/MS under positive mode withmultiple reaction monitoring(MRM). The final results showed that the linear range ofAuramine O, Rhodamine B was0.2~10ng·mL-1and that of Chrysoidine, Para Red,Sudan I~IV was2~100ng·mL-1. The accuracy of the chili products was96.00%~112.50%. The relative standard deviation (RSD) of intra-and inter-dayprecision were0.20%~14.14%. The Limit of quantification (LOQ, S/N≥10) and lirnitof detection (LOD, S/N≥3) were in the range of0.60~6.00μg·kg-1and0.15~1.20μg·kg-1. Average recoveries of six different kinds of chilli products at three addingstandard levels were60.00%~118.75%. Method validation showed that this method isaccurate and reliable. 2. A LC-MS/MS method for simultaneou s determination of eight industry dyesin bean products was proposed.The final quantitative method are as follows:The bean products were extracted with acetonitrile and assisted with ultrasound.The target analytes were separated on a C18column with gradient elution using5mmol/L ammonium acetate solution containing0.1%formic acid and methanol asmobile phase, and then detected by MS/MS under positive mode with multiplereaction monitoring(MRM). The final results showed that the linear range ofAuramine O, Rhodamine B was0.2~10ng·mL-1and that of Chrysoidine, Para Red,Sudan I~IV was2~100ng·mL-1. The accuracy of the bean products was91.40%~107.60%. The RSD of intra-and inter-day precision were3.11%~14.77%.The LOQ and LOD were in the range of0.20~4.00μg·kg-1and0.06~1.50μg·kg-1.Average recoveries of six different kinds of bean products at three adding standardlevels were59.73%~116.91%. Method validation showed that this method is accurateand reliable.3. The method was ued to analyte food samples from the market.40samples were collected from market, and Rhodamine B was found in1sample, Rhodamine B and Chrysoidine were found in another sample.