| Fow-injection chemiluminescence (FI-CL) method has the advantages of high sensitivity, rapaid, low cost and wide linear range, and has received much attention by analysts. This research method has been widely applied in pharmaceuticals, food, environmental sciences and clinical analysis. Luminol as one of the traditional chemiluminescence reagents, has the characteristics of good water-solubility, high quantum yield, and easy to synthesis. Therefore, researching the sensitizers of luninol chemiluminescence system to extend the application of this system is necessary. It was found that CdTe QDs have stronger catalysis ability on luminol-KMnO4system, and we established a new luminol-KMnO4-CdTe QDs chemiluminescence system. And the new CL systems of luminol-KMnO4-CdTe QDs were applied to detect2-methoxyestradiol and ferulic acid. In this research we developed a new metal ion catalyst of CdCl2on the luminol-K3Fe(CN)6CL system. The possible mechanism of luminol-K3Fe(CN)6-CdCl2system is briefly recommended. The new system has been successfully applied for the determination of caffeic aicd in pharmaceutical preparation and plasma samples. The using of sensitizer not only improve the sensitivity of the luminol chemiluminescence system, but also promote the study of the new luminol chemiluminescence system, which further extend the application of flow-injection chemiluminescence method. The abstract of this research is showed as follows:1. Based on the strong sensibilization effect of CdTe QDs on luminol-KMnO4CL system, build luminol-KMnO4-CdTe QDs chemiluminescence system for the determination of2-methoxyestradiol.In this study, the CdTe QDs was synthesised, and it was found that CdTe Quantum dots (QDs) could observably enhance the signal intensity of luminol-KMnO4reaction and based on this observation, luminol-KMnO4-CdTe QDs was established. Based on the inhibition on the luminol-KMnO4-CdTe QDs system, a feasible method was established for the detection of2-ME with satisfactory analysis results. The corresponding linear range was9.0×10-10-8.0×10-8g/ml and the quantitation limit was3.06×10-10g/ml. There was no significant difference between the results of this method and that of high performance liquid chromatography (HPLC). In addition, the possible reaction mechanism of the system was also discussed preliminary.2. Determination of ferulic acid based on inhibition of the luminol-KMnO4-CdTe QDs reaction.In the present study, it was found that ferulic acid could strongly weaken the CL signal intensity of luminol-KMnO4-CdTe QDs system and based on this phenomenon, a new CL method was established for the convenient analysis of ferulic acid with satisfactory detection results. The detection limit for this method was1.9×10-10g/ml, linear range was5.0×10-10-4.0×10-8g/ml with good linear relationship. This method was suitable for the analysis of the ferulic acid biological samples due to the characteristic of rapaid and convenient.3. Based on the strong sensibilization effect of CdCl2on luminol-K3Fe(CN)6CL system, build a new luminol-K3Fe(CN)6-CdCl2chemiluminescence system for the determination of caffeic acid.It was found that Cd2+could observably enhance the CL reaction of luminol-K3Fe(CN)6, as a raw material of the synthesis of CdTe QDs, and based on this catalysis, the novel system of luminol-K3Fe(CN)6-CdCl2was established. Based on the inhibition on the luminol-K3Fe(CN)6-CdCl2system, a feasible method was established for the detection of caffeic acid with satisfactory analysis results. The detection limit of this method was2.52×10-10g/ml for the plasma sample of caffeic acid, and the method had good selectivity, high sensitivity and could be applied to determine the caffeic acid. In addition, the potential mechanism of this reaction was discussed preliminary as well. |
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