The Study On The Self-assembly And Protein Adsorption Kinetic Of Amphipathic Block Copolymers

In the recent ten years, nanotechnology has vigorously promoted the development of various fields. Molecular self-assembly, as a method for preparing nanomaterials, have received scientists’widespread attention and meantime the block copolymer, because of its obvious advantages in the self-assembly process, has gained more attention than before. This paper, based on the combination of experiments and theories, using the self-assembly of amphipathic block copolymers to discuss several factors affecting the polymer micelles morphology and making regulations through appropriate methods. At the same time, it also utilizes quartz crystal microbalance to investigate deeply the dynamic process of the interactions between protein and the polymer surface. The specific results are summarized as follows:1. First this paper, using several kinds of amphipathic block copolymers, adopts polymer film rehydration, direct dissolution and blending methods respectively to prepare some micelle aggregates of different forms such as spherical, clubbed and vesicular shapes. Meanwhile it utilizes transmission electron microscopy (TEM) to observe and study the effects of conditions like blending time and blending ratio of block copolymer micelles on micelle morphology. Finally through experiment regulation a kind of biodegradable polymer vesicle with larger particle size.2. This paper, using the quartz crystal microbalance (QCM-D) with dissipation function, focuses on the interaction mechanism between structure-controllable surface and protein as well as adsorption kinetics, revealing the impacts of materials’ chemical compositions, grafting density and surface morphology on protein and adsorption behavior, showing the variation of protein conformation in the adsorption process and the law of its dependence on material compositions and structures, and building the relationship between surface structure, protein adsorption and blood compatibility.The above work illustrates that when using amphipathic block copolymers to prepare polymer micelles, the blending ration and time of polymers exert impact on its morphology. This experiment not only obtains the biocompatible polymer vesicles through regulation but discusses the action mechanism between polymer materials and proteins under physiological environment. It provides guiding suggestions for people’s further design and development of artificial membrane structure.