Extract Oleanolic Acid From Chaenomeles Sinesis Based On Molecular Imprinting Technique

Chaenomeles sinensis Koehne is a collection of edible and medicinal plant since ancient times. The usage and development of the Chaenomeles sinensis Koehne resource has been paid people’s further attention, with the production of the Chaenomeles sinensis Koehne increased and the improvement of the people’s living requirements. It has converted from the traditional use as enjoy and edible plant to comprehensive processing products in producing and development of Chaenomeles sinensis Koehne. Certain new production (Papaya wine papaya slices and papaya drink) have been presented in the market,which provide a new thought for comprehensive utilization and value increase. This research pays attention to extracting oleanolic acid, the component with health care function in the resource of the papaya,uses a new technology-molecular imprinting technique,which was widely studyed and researched, combinate with high performance liquid chromatograpHy, prepare the oleanolic acid molecularly imprinted polymer, separate and purify selectively the oleanolic in the papaya light crude extract, achieve the purpose of improving oleanolic acid extraction yield and provide a new thought and way for the comprehensive utilization of light papaya. The main experimental results are as follows:1. Use molecularly imprinted polymerization technology, in the prepareation of oleanolic acid molecularly imprinted polymer, changing propotion of the template molecule and function monomer, the polymerization of monomers and crosslinking agent,and the chosen and the proportion of the initiator. Optimize the synthesis conditions which include polymerization temperature and time to get certain polymer with rigidity and toughness. The optimum synthesis condition has been choose:oleanolic acid:2-crotonic acid1:3, styrene: divinylbenzene2:1, the initiator (sodium persulfate)4%, the best synthesis temperature:85℃, the best synthesis time:30h, the best eluate is10%acetic acid methanol solution.2. In order to verify the synthesis of molecularly imprinted polymerhas a good adsorption performance to the oleanolic acid, the experiment also verify the characterization of molecularly imprinted polymer experiment. The characterization of the experiment is infrared spectrum scanning, specific adsorption experiments, adsorption equilibrium binding experiments and adsorption kinetics experiment,respectively. At the same time, the experiment also optimize the elution condition of template and use two aspects-elution volume ratio and the elution volume to demonstrate the experiment. Finally it is concluded that between template molecules and functional monomer have the formation of hydrogen bonds in the synthesis molecularly imprinted polymer by scanning infrared spectrum. Molecularly imprinted polymer compared with non-molecularly imprinted polymer has obvous advantages in adsorption capacity to oleanolic acid. Molecularly imprinted polymer for0.05mg/mL OA standard solution binding capacity is0.98mg/g, while, the UA is0.19mg/g.3. Extract oleanolic acid crudely, use response surface method for experiment design, set about the experiment from three aspects:liquid ratio, extraction time, temperature, research the effect by using ultrasonic method to extract the oleanolic, and determine the best ectraction conditions. When the temperature is50℃, liquid ratio is5.0, the extraction time is2h, the additive amount of cellulase is0.32%.The yield of OA is3.7mg/g.4. Use the olecularly oleanolic acid olecularly imprinted polymer to adsorb the papaya oleanolic acid in crude extract specificly. Optimize the injector and eluent conditions. The best condition:8mL8%acetic acid-methanol solution leaching,removing impurities outside of olean olic acie,10mL10%acetic acid-methanol solution for elution, the purity of OA is89.45%.