Preparation Of Magnetic Graph Ene Oxide And The Application As A Drug Carrier

Graphene oxide(GO), a novel single-atom-thick sheet, which is composed ofsp2hybridized carbon atoms with disordered oxidised sp3domains as well as defectsof carbon vacancies surrounded in a two-dimensional hexagonal lattice. Thefunctional groups, including hydroxyl, carboxyl and epoxy groups on the surface ofGO, give it good water-solubility and biocompatibility, and can be modified forvarious derivatives. In addition, GO has a large number of sp2carbon atoms, largeplanar surface area, so it has been widely applied as nanocomposites for drugdelivery in medicine research.In this paper, firstly, iron oxide nanoparticles(IONP) were chemically depositedonto the surface of GO through hydrothermal coprecipitation, which were thenfunctionalized with water-soluble PEI through amide condensation reaction, toobtain water-soluble magnetic targeting carrier GO-IONP-PEI. And it wascharacterized by thermal gravimetric analysis(TGA), transmission electronmicroscopy(TEM), fourier transform infrared spectroscopy(FT-IR), dynamic laserlight scattering (DLS) and so on. The results showed that GO-IONP-PEI carrier wassuccessfully constructed and had good biocompatibility, high aqueous solubility,stability，excellent magnetic properties and an even size distribution.Then, doxorubicin was loaded onto the GO-IONP-PEI, forming magnetictargeting drug delivery system. The single factor experiments concerning the ratio ofcarrier and DOX, ultrasonic time and other factors were carried to investigate theimpact on loading capacity, and the best preparation technology was determined.And it was characterized by UV spectrophotometer, FT-IR and so on. The averageparticle size of GO-IONP-PEI/DOX was220±2.1nm, average zeta potential was41.75±1.5mV. In this experiment, the drug loading was up to100%.Finally, the constructed tumor magnetic targeting preparationGO-IONP-PEI/DOX was investigated with human breast cancer MCF-7cells invitro. The results showed that GO-IONP-PEI carrier was non-toxic to MCF-7cells,while GO-IONP-PEI/DOX inhibited the growth of cells obviously, and this inhibition was time and dose dependent. GO-IONP-PEI and GO-IONP-PEI/DOXcould effectively transfer into MCF-7cells. In addition, in vitro magnetic targetingexperiments, the preparation was targeting to gather in magnetic field, and thenumber of surviving cells near magnetic field was far less than that far away frommagnetic field or in non-magnetic field, showing that the preparation had a goodmagnetic targeting property.