The Study Of Antibiotics In Fish Based On Surface-Enhanced Raman Spectroscopy Technology

Since antibiotics have been discovered, were used widely by humans, includingfor the prevention and control of all kinds of bacterial disease of human beings,livestock and aquatic products.But humans tend to ignore the dangers of excessiveuse of antibiotics. Recent years, more and more food problems caused by antibioticresidues begin to be reported, as a result of which, people became aware of thedangers of antibiotics. At present, a lot of countries and regions have formulatedrelated policies, to restrict or prohibit the use of antibiotics. Therefore, realizing thedetection of antibiotic residues will provide strong support for this phenomenon.At present the methods of detecting antibiotic residues main include bioassaymethod, immunoassay and physical and chemical analysis. But these methods havetheir own defects, so they were limited certainly in use. In this paper, the detection ofantibiotics using surface-enhanced Raman spectroscopy （SERS）.This detectionmethod can detect to the level of the single molecule, realizing nondestructive, simple,rapid, high accuracy and high sensitivity detection.This paper used SERS methods to detect antibiotics in fish. Selected four kindsof antibiotics(sulfamerazine, Sulfamethazine, ciprofloxacin enrofloxacin) that wererestricted by the state, mixed with aquatic products (fish) and extracted. Through themethod above, we can explore a most stable and effective method to extract theantibiotics in aquatic products.Based on the surface enhanced nano substrate ofsensitive and owning antibiotics adsorption properties that was preparated by our ownresearch group before,to detect and analysis the Raman spectroscopy of extractiveantibiotics.In experiment, an agarose-silver enhanced substrate was first developed.. Bycomparing the results of532nm and785nm detection system, discovered that whenusing silver sol film as enhanced substrate, glasses can replace quartz plate in532nmdetective system. As for785nm detective system,if plating a layer of agarose on thesurface of glasses, also can also achieve the same detective results as quartz plate.This can saves the experimental cost effectively. Methanol, acetonitrile and ethyl acetate were respectively used as solvent toequipped with each antibiotic to standard solution, and then study the effect onantibiotic detection of three kinds of solvent; Mixed fish meat with antibiotics, usingthree kinds of solvents as extracting agent, to extract the antibiotics from fish,compared the difference of the three kinds of extraction agent to the detection ofantibiotics in fish. The results showed that, solvent has significant effect on spectraldetection of antibiotic standard liquid. Detecting the extracting solution of the mixtureof antibiotics and fish, founded that the substance in fish has a great interference ondetection of antibiotics.And the results obtained with different extraction agents arealso different; By comparison and analysis, ultimately determined that ethyl acetatewas best as extraction agent.And by repeating experiment and research, has exploredthe suitable extractive method for each antibiotic.The silver sol and silver sol membrane were respectively used as enhancedsubstrate to test the samples. But in the comparison found that the stability of thesilver sol membrane is worse than the silver sol. By using silver sol as enhancedsubstrate to detect the solution of enrofloxacin,ciprofloxacin, sulfamerazine andsulfamethazine.To the first two antibiotics, the minimum detectable concentrationscan achieve60ppb and30ppb respectively. For the latter two antibiotics,theminimum detectable concentrations are2ppm.The minimum detectable concentrationsof extracting solution of fish and enrofloxacin, sulfamerazine and sulfamethazine are200ppb,1ppm and0.5ppm respectively. There have not find the literatures aboutdetection of these three antibiotics in fish using the method of SERS at present.