The Effects Of Glycoprotein From Jellyfish (R.e) On The MRNA Expression Of The Cytokines

Jellyfish (Rhopilema esculehtum) is a species medicinal and edible large jellyfish,which having very high economic, nutritional and medicinal value. Glycoprotein is acomplex that made of glucose and protein，widely exists in organisms, as a role of theregulation and recognition in cell, and having a variety of biological activity, such asantitumor activity, immune activity and so on. Cytokines are small moleculesglycoprotein or protein, which can transfer information between cells, and it is animportant component of the immune system. This paper focuses on the effect of theglycoprotein from jellyfish on the mRNA expression of cytokines, the immune activityof the glycoprotein from jellyfish were analyzed at the molecular level, The purpose isto provide a theoretical basis for the development and utilization of jellyfish resources,and provide a theoretical reference for the development of health food and newimmune drugs. In this paper, the research contents and results are as follows:1. In vitro experiments: The lymphocytes were prepared from the spleen of miceand divided into five groups (normal control group, model control group, jellyfishglycoprotein high, medium and low dose group), placed in a cell incubator60h. Then,total RNA was extracted cells in each group, reverse transcription of cDNA, thefluorescent quantitative real-time polymerase chain reaction technique was used toanalyze mRNA relative expression of Th1, Th2and Th17cytokines in each group. Theexperimental data are processed using the relative quantification method. Resultsshowed that: Through contrast experimental data，we got the jellyfish glycoproteincould promot Th1cytokines secretion and inhibit Th2and Th17cytokines secretion.2. In vivo experiments:36healthy Bal B/C mice (SPF,18~22g) were randomlydivided into6groups of9mice each: normal control group, model control group,positive control group, low dose (25mg/kg) group, middle dose (50mg/kg) group andhigh dose (100mg/kg) groups, respectively. After21days treatment period, lymphocytes were prepared from the spleen of mice and total RNA was isolated. Then,everse transcription of cDNA, the fluorescent quantitative real-time polymerase chainreaction technique was used to analyze mRNA relative expression of Th1, Th2andTh17cytokines in each group. The experimental data are processed using the relativequantification method. Results showed that: Through contrast experimental data，wegot the jellyfish glycoprotein could promot Th1cytokines secretion and inhibit Th2and Th17cytokines secretion.3. The results of in vivo and vitro experiments showed, the influence trend of thecytokine mRNA expression is mostly consistent. We suggested the glycoprotein fromjellyfish may promot the secretion of Th1cytokines, and inhibit the role of Th2andTh17cytokines, which have immunomodulatory activity.