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Toxicities Of Ago Dyes To Saccharomyces Cerevisiae

Posted on:2013-08-11Degree:MasterType:Thesis
Country:ChinaCandidate:L GaoFull Text:PDF
GTID:2181330362963347Subject:Environmental Engineering
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With the development of dyes and the dyeing and printing industry, dyeingindustry had become one of the largest polluter on the planet. As a big country ofproducing dyes,the dye wastewater discharge capacity has reach 0.157 billion m3/a.Ina variety of dyes,azo dyes is used widest,which makes its wastewater emissionshuge.Select the appropriate representation of biological contaminant toxicity is one ofthe most important means of ecological risk assessment.Study in this area,it can laythe foundation for the ecological risk assessment of azo dyes,and it also can providereliable basis for the management in the space of setting pollution control standard.In the study,we used saccharomyces cerevisiae as eukaryotic model organisms toestablish the method to determine the azo dyes toxicity.The replicative life span ofsaccharomyces cerevisiae used to reflect the sub-acute toxicity,the chronological lifespan used to reflect the chronic toxicity,and the teratogentic and carcinogentic effectsis reflect by counting the ratio of different types dead cells. The result reflect thatchronic toxicity is Direct Purple 1> Direct Blue 15> Direct Brown RN >Direct Yellow 50;sub-acute toxicity is Direct Yellow 50> Direct Blue 15> Direct Brown RN > Direct Purple 1;the teratogentic and carcinogentic effects is Direct Brown RN > Direct Purple 1> DirectBlue 15>Direct Yellow 50.Moreover,the toxicity increase with the dye concentration.
Keywords/Search Tags:Saccharomyces cerevisiae, Azo dyes, Toxicity effect, Replicative life span, Chronological life span
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