| In this paper, we studied the effect of cadmium poisoning on various kinds of tissues,physiological metabolism, MT dynamic expression and MT gene transcribe of Pelteobagrusfulvidraco. We placed the healthy P. fulvidracos in 0.05 ,0.5 ,1 mg/L of cadmium solution,and measured sampling different indexs at 6 h, 12 h, 24 h, 48 h, 72 h, 96 h, 144 h. Theexperimental results are as follows.1 The cadmium accumulation in the gut is much higher than the gills, gill cover, muscleand skin. The cadmium accumulation in the liver and kidney is the highest. Gill cadmiumaccumulation is high in addition to internal organs. Gill cover, muscle and skin cadmiumaccumulation is always very low. All organizations, especially the liver, kidney and gillcadmium accumulation increased with cadmium concentration increased, with the exposuretime the extension increases, showing a clear dose-effect and time-effect relationship.2 In the serum, the activity of LDH was decreased,while the activity of ACP wassignificantly increased, AKP activity at 0.05 mg/L cadmium solution were significantlyincreased, and it’s just like parabola under high concentrations cadmium stress. The activitiesof AKP and ACP of gills were downward trend, and were always lower than the control group.The activities of AKP and ACP of liver were significantly decreased in the high concentrationgroup. AKP was activated early while after 24 h was strongly inhibited, and that is the typicalHormesis phenomenon. AKP activity in the kidney under high concentrations cadmium stresswas inhibited, and it was significantly lower than the control group. The activities of AKPand ACP of kidney were significantly decreased. In the muscle, the activity of CAT and LDHchanged liake waveform, and the activities of AKP and ACP were significantly increased.The results showed that concentrations of cadmium, P. fulvidraco body tissues by regulatingthe activity of different enzymes to maintain the steady-state internal environment, but whenthe cadmium concentration and exposure time beyond the scope of the body metabolicactivity under varying degrees of inhibition; heavy metal cadmium on the normalphysiological metabolism of P. fulvidraco had a serious impact on.3 The order of the metallothionein background content is liver> kidney> gill filaments. Under the cadmium stress, the metallothionein content of the 3 organizations were increasedand then stabilized, which show that cadmium can induce metallothionein synthesis andexpression. The inducement mainly occured in the 0-12 h; and the inducement of the highconcentration group was stronger than the low concentration group. The expression ofmetallothionein and cadmium concentration in water is a positive correlation. Liver andkidney are the preferred target tissues to evaluate of cadmium pollution.4 According to MT gene of liver semi-quantitative RT-PCR experiments, with theincrease of cadmium concentration, the expression of hepatic MT was significantly increased.The hepatic MT expression increased rapidly in the early exposure, and there was asignificant difference between the 1 mg/L cadmium group and the control group at 12 h. Afterexposure of 12 h, there was a slight decrease of MT expression, and it maintained this level ofbalance. The results showed that the metallothionein gene expression analysis can be used asa pollution indicator on the early detection of cadmium.In summary, cadmium lead P. fulvidraco gult and gill accumulated a large number ofcadmium, and showed a dose-response and time-effect relationship. Under cadmium stress,many kinds of enzymes of P. fulvidraco were abnormal, showing that cadmium interfered thebody’s normal physicological metabolism. P. fulvidraco produced a large number of MTinduced by cadmium. That may be an emergency strategies P. fulvidraco taken in response toshort-term heavy metal toxicity. |
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