Primary Analysis Of Rice Mutant Fly And Lla

GH3 was first identified as a IAA conjugate enzyme in soybean (Glycine max) and are responsible for IAA homeostasis. There are 14 members in rice and more than 20 members in Arabidopsis in this family. Studies on GH3 gene functions in Arabidopsis and soybeans were reported. GH3 protein can synthesize IAA–amino acid conjugated.We detected a rice mutant-fly. And it shows a dwarf phenotype. The number of lateral roots of fly is much fewer, its seeds are thin and slender. Paraffin slices of lamina joint, leaf blade and stem reveal that fly has more xylem cells in lamina joint and more parenchyma cells in leaf and stem. Moreover, fly has less vascular bundles and thinner stem than WT.7 day seedlings of WT and fly treated with different concentration of IAA show that 0.1pMol IAA can stimulate WT shoot growth , while 0.5pMol IAA can stimulate fly shoot growth. fly is less sensitive to exogenous IAA. GH3-GST fusion protein was expressed in E.coli and purified and its enzyme activity acting to conjugate IAA has been tested in vitro.A large-leaf-angle and semi-dwarf mutant, named lla, was found when transforming rice with a RNAi construct. RT-PCR indicates that the RNA level in mutant did not change too much. Southern blot gives another evidence that the mutant is a single T-DNA insertion line. T1 individuals show just the same phenotype with T0.TAIL-PCR is performed to isolate the specific gene.