Transcriptome Of Seed And DGE Method Research Of Seed Low Temperature Germination Period In Lepidium Apetalum Willd

Lepidium apetalum Willd belongs to Brassicaceae Lepidium L, and is widely distributed. They can adapt to wide range of temperature. Especially, Lepidium apetalum Willd, which grow in Northern part of Xinjiang region, are typical ephermerd plants. They have to toleranted to low temperature in early spring why are winder seed germination and seedling growth.Temperature is one of the most important environmental factors limiting geographical distribution of plants. Also, it can affect seed germination significanthy. So studying molecular mechanisms of seed germination, under low temperature, had important values to basin research and solving practical production problems. Form previous research, the stay on could be broken after of Lepidium seed germination a short period of high temperature treatment. But, the mechanism was not clearly. So transcriptome of Lepidium apetalum under different phase of seed germination were sequenced and analysed. Also, different expressing genes were identified by DGE analysis, which may be reateal to break the stagnation of Lepidium seed germination under low temperature by a short period of high temperature treatment. The main results were listed as follows:(1) In this study, the transcriptome of Lepidium apetalum was sequenced using the Illumina HiSeqTM 2000 sequencing platform. The clean reads were then de novo assembled into 40303 unigenes. Among has 27935 unigenes were annotated by a similarity search against seen public databases. The results showed that 534 genes were assigned to second metabolic pathway. Using the software of Lepidium seed transcriptome of SSR and SNP polymorphism analysis, after detecting 40304 unigenes, a total of 6304 SSRs and 127274 SNPs were identified from the sequence of transcription.(2) In this study, 1089 transcription factors were identified and classified into 26 different common families by searching unique transcripts. It was showed that 534 genes were assigned to second metabolic pathway.(3) Through comparing gene expressing level between stagnation phenomenon of low temperature of seed germination and break low temperature of seed stagnation germination seed expressed genes, we obtained 2108 differentially expression genes. The differentially expression genes enriched in pathway related transcription factor, ribonucleotide metabolic process and terpenoid biosynthesis as well as plant hormone signal transduction.(4) Full length cDNA of transcription factor LaAP2, LaDREB, LaBBX were cloned, which their expression level up-regulated significantly. And the expression of LaAP2 up-regulated in the break stagnation phenomenon after low temperature of seed germination, This may be related to seed break stagnation phenomenon of low temperature germination.In the study we got Transcriptome of Seed and DGE method research of seed low temperature germination period in Lepidium apetalum Willd, and obtained Three transcription factors related to seed break stagnation phenomenon of low temperature germination. Enrich the research content of Lepidium apetalum Willd transcriptomics and transcription factors. These study will contribute to study of the molecular mechanism of Lepidium apetalum Willd seed low temperature germination process on genetics and molecular biology.