Research On The Bacterial Community Of Sulfur-cold Spring In Urumqi Response To Earthquake

Investigate bacterial community compositions and diversity in the Urumqi sulfur-cold spring during the monitoring period; Explore the reflection between the changes of sulfur-cold spring bacterial community diversity and the hydro geochemical factors; Analysis the changes of sulfur-cold spring bacterial community diversity response to the earthquake. Methods: modern molecular biology techniques T-RFLP and the real-time fluorescence quantitative PCR technology were used to analysis the Urumqi sulfur-cold spring bacteria community compositions and diversity changes response to the biogeochemical elements and earthquake during the monitoring period. Results: T-RFLP technology analysis result we can find that Urumqi sulfur-cold spring bacterial community structure dominant groups contain Firmicutes(fragment representative size 90bp), Proteobacteria(129bp and 454bp) and Spirochaetes(304bp).Of them, the fragment representative size 90 bp and 129 bp bacteria genera were always stable in the detection period, while the represented fragment size 304 bp and 454 bp were intermittently disappeared. In addition, we could also calculated the bacterial community structure diversity index, and we conclude that the change of the content of hydrogen sulfide with the Shannon-Wiener index(H) showed a significant negative correlation, as well as the Simpson index(D) showed a significant negative correlation; Shannon-Wiener index(H) in pre and post earthquake has obvious changes, but there is no greater volatility in the Simpson index(D) and Evenness index(E) before and after the earthquake. By real-time fluorescence quantitative PCR instrument detected that CO2 content changes in the sulfur-cold spring microbial total DNA content change and hydrogen chemical factor CO2 content has significant positive correlation, and has significant negative correlation with HCO3-.The change of total DNA concentration of sulfur-cold spring was higher than that of the post earthquake, and the maximum recovery value increased with the increase of magnitude; The total DNA concentration of the spring water in the 5-17 days after the earthquake occurred, which had a great value, and this change is closely associated with change. Conclusion: Urumqi Sulfur-cold spring bacterial community structure diversity index is not significant in the detection period, but the Shannon-Wiener index(H) and Simpson index(D) changes were sensitive to the hydrological physicochemical factors HS-. Meanwhile, The Shannon-Wiener index(H) was also sensitive to the earthquake. There are significant positive correlation and negative correlation between CO2 and HCO3- in the density of bacterial community and the hydrological physical and chemical factors of spring water, and it has a certain correlation with the earthquake.