Study Of Micro-volume Dynamic Hybridization Device

Nucleic acid molecular hybridization has become one of the most widely used techniques of biochemistry, molecular biology and cell biology as an important means of nucleic acid detection technology.In the past few years, along with study on nanometric materials in depth, the researchers found that nucleic acid hybridization reaction applying magnetic nanoparticles as the solid-phase carriers has significant advantages over the traditional hybridization methods in terms of separation and purification, but the reaction efficiency may be affected due to the magnetic particle sedimentation and steric hindrance resulting from the dimensional space surface restrictions. Therefore, effective way is to make the magnetic particles suspended in the reaction in order to take full advantage of the magnetic particles and overcome their disadvantages. It is necessary to develop an automation device to fully mix and disturb magnetic particles in a micro-volume hybridization reaction system, but there is still a lack of development and application report in this regard. Based on the above requirements, thesis is aimed at developing a better dynamic hybrid device which is suitable for magnetic nanoparticles as solid-phase carriers.Firstly, we checked the functions and parameters needed of the system, then determined the function module of dynamic micro volume hybridization instrument, including the accurate temperature control module, mixing module, the core control module, human-computer interaction module and other auxiliary modules. We use incremental PID and PWM to realize the accurate control of temperature. It has the characteristics of faster rate of temperature increase, fine temperature homogeneity, and ease of controlling. The developed mixing device is compact and flexible. The three-dimensional rotate speed can be adjusted in 0-40rpm. User can set parameter through the touch screen, possessing the merits of simple and comfortable.Finally, according to the system design objectives, relevant experiments have been performed to validate the scientificity, rationality and practicality of the device. The system can not only provide temperature environment suitable for nucleic acid hybridization, but also can realize mixing of the magnetic nanoparticles. The results of hybridization experiments also show that this system can improve the efficiency of hybridization greatly.