Response To Drought Stress And Drought Induced Protein In Faba Bean At Germination And Seedling Stages

Faba bean is minor crops in the northwest region, which is growed in Qinghai because it can improve the soil structure through nitrogen-fixing root nodules and increase the farmers, income. With the area structure adjustment, Faba bean production areas transfer from the irrigation agriculture areas to the rainfed farming. Cultivating faba beans with strong drought resistance is vital significant to improve dry areas comprehensive production capacity and promote the sustainable development of faba bean. This article evaluat drought resistance of different faba beans by the method of artificial simulated drought stress on germination and seedling and different proteins of drought-resistant cultivar are analysised by two-dimensional gel electrophoresis to reveal the drought resistance mechanism of faba bean and provide the basis for breeding drought-resistant cultivar, the results are as follows:1.We evaluat drought resistance of 9 faba bean varieties in seed germination by PEG hypertonic solution and in seedling by potted plants combined with the artificial water-control, verify the result by using field experiment. we find that Gadadou, Qinghai No.13, Lincan No.6 and Qinghai No.12 are moderate drought resistance’s; 5 faba bean varieties are drought resistance’s. The indexes of drought resistance identification are Germination index, germinative force, Lateral root length and radical dry weight, and 15% is drought optimal concentration of PEG in seeds germination. At the same time, we find faba bean with tall plant height, high density of lateral root, higher chlorophyll content and lower malondialdehyde content in seedling has strong drought resistance.2. Drought-resistant cultivar are analysised by two-dimensional gel electrophoresis. We find 32 differentially expressed proteins, including 27 protein points show down-regulated in the plant of drought stress, the protein points decrease more than four times are: stress-inducible protein, PGMand hypothetical protein. 5 protein points show up-regulated in the plant of drought stress, the protein points only expressed under the drought stress are: glutamate-glyoxylate aminotransferaseand proteasome subunit alpha type-7-A protein, shows that drought stress could suppress certain plant protein synthesis and produce some new protein induced to resist the impact of the drought.3. Different protein points are analyzed by MALDI- TOF/TOF mass spectrometry identification, the results show that 30 different protein points were divided into five types by metabolic pathway and biological function, 40.6% were regulatory proteins, Metabolism and energy were 28%, other protein were 15.6%, cytoskeleton and unknown protein was 3.1% and 9.3%. Regulatory proteins are main heat shock protein helpping degrade misfolded proteins to resist drought stress. Regulation of energy metabolism was mainly through some photosynthetic related proteins(putative rubisco subunit binding-protein alpha subunit、glutamate-glyoxylate aminotransferase 、 D-alanyl-D-alanine-endopeptidase) to adapt to drought stress. KEEG Pathway enrichment analysis find Protein processing in endoplasmic reticulum reached extremely significant difference among them(P<0.01),Amino sugar and nucleotide sugar metabolism、Glycine, serine and threonine metabolism、Glyoxylate and dicarboxylate metabolism and Thiamine metabolis reached significant difference among them(P<0.05).