Comparative Study On Subgalical Sediments In Tianshan No.1 Glacier Based On The Different Biological Information Processes

Purpose: The glacier belongs to the important component of freshwater ecosystem throughout the world. There is a large amount of pronucleus bacteria and eukaryon microorganism in glacier system. Its found that the former research of glacier biosystem mainly concentrated on bacteria relevant theory while the research on fungus in glacier is less. By taking the settled layer at the bottom of glacier, this article carried out comparison study of diversification and community structure by combining with the fungus in glacier surface dust samples. It was analyzed that the fungus diversification, community structure and phylogeny in the surface cryoconite and bottom settled layer of Tianshan No.1 glacier through different information flow process, and revealed the differences and similarities between fungus communities at bottom settled layer, which provided reference and foundation for the diversification as well as community structure researches of fungus at bottom settled layer of Tianshan No.1 glacier.Methods: 1) The clone library Adopted fungus ITS regional specific primers was amplify and constructed, and classified OUT and counted diversification based on sequence similarity, selected representative OUT sequence and constructed phylogenetic tree after comparing with NCBI and RDP database so as to carry out analytical statistics on community structure. 2)Adopted fungus ITS regional specific primers to amplify PCR,amplified the primer pair ITS and delivered the sample obtained after processing to Shanghai Majorbio Co., Ltd. to carry out high-throughput sequencing by using Illmina Miseq sequencing platform; A set of optimal flow for processing glacier fungus samples were obtained after analyzing the processed data.Results: tianshan mountain glacier no. 1 in the dust on the surface of the ice fungi taxa belonging to the five systems development groups, including basidiomycete fungi(Basidiomycota), ascomycetes door(Ascomycota), chytrid fungus door(Chytridiomycota),Monoblepharidomycota and Chlorophyta(Chlorophyta) fungi;Sediments at the bottom of the door(Basidiomycota), fungi belonging to basidiomycete fungi ascomycetes door(Ascomycota), chytrid fungus(Chytridiomycota) three system development groups,including Basidiomycota, Ascomycota door of fungi is dust tianshan no.1 glacier ice on the surface of sediments and the advantages of bacteria, but in the level two distinct advantage bacterium, environmental samples in the dust on the surface of the ice Leucosporidium,Tetracladium, Aspergillus, Rhizophydium, Rhodotorula abundance is higher, and the bottom sediments Simplicillium, Aspergillus, Phoma, Cladosporium relative abundance is higher.The steps of information flow that finally determined through the comparison analysis of different information flows: a) quality control of original data to be completed by sequencing company through using BIPES pipeline; b) sub-package of samples to be finished by sequencing company through using the perl script developed by itself; c) the splice of sequence to be completed by using NW algorithm; d) carry out clustering analysis and implement the annotation of clustering and classified information by using the qiime platform built-in perl script; e) diversification analysis, use the qiime platform built-in perl script to calculate index and apply R language to draw chart and modify with photoshop software subsequently; f) statistics of community structure, carry out statistics on gross samples separately at Phylum level and category level.Conclusion: The diversification of fungus class group in surface cryoconite of glacier is higher than that at bottom settled layer; The fungus class groups in two environments are similar at gate level; However, at category level, there were great difference of the fungus class group and phylogenetic analysis respectively.