The Response Of Tobacco BY-2 Cells On Growth And Respiration Under Acid Rain

Atmospheric condition is the main factor for human life and human activity.As the development of industrialization and urbanization has accelerated,the quantity demanded of the traffic and petroleum energy has a sharp increase,growth in living standard,material is used up that has increased,when human release pollutants into the environment,it will polluted the atmospheric.It will have the pressure and threat on human health, environment and social economy, especially the SO2 and NOx, so the times of H2SO4, HNO3 and HNO2 acid rain have increased by year,the strength has increased year by year, acid rain not noly has damage for the construction,also has influence on the human and animals.To study the influence of acid rain on cell growth and cellular respiration of the plant,but without the influence of the Chloroplast metabolism,the article uses the Bright Yellow-2 (BY-2) cells that only with mitochondria are for the studying material,simulate different pHs (1.5、2.5、3.5、4.5、5.5) of SAR、NAR and MAR to treat the cells,so to know the influence of cell growth、cellular respiration、 enzymatic activity and cell activity.the greater the acidity,the more stress of Tobacco BY-2 cells, the results are below:1. Simulate different types acid rain,the dry weight of Tobacco BY-2 cells has reduced,as the pH has reduced,the more dry weight of tobacco BY-2 cells has fallen,the stress of SAR is the maximum,then the NAR, the dry weight of MAR has fallen,but is lessthan SAR. So the acid rain solution can hurt the Tobacco BY-2 cells,the acid rain has blocked the physiology and growth of Tobacco BY-2 cells.2. Simulate different types acid rain, cellular respiration of Tobacco BY-2 cells has under the influence,under the strong acid(pH=1.5), cellular respiration is less,the strong acid rain can control the cellular respiration,but cellular respiration has increase or fallen in other treatment groups, cellular respiration apper mess,so the cells have meet the acid rain stress,and the acid rain solution can hurt the Tobacco BY-2 cells.3. Simulate different types acid rain, enzymatic activity of Tobacco BY-2 cells has under the influence,the CAT activity is less than the normal cells,the SOD and POD activitys has increased by time,and under the stong acid(pH=1.5),he MDA is great,and has increased,but the MD A is less in other treatment groups.4. Simulate different types acid rain,the cell activity of Tobacco BY-2 cells has under the influence,under the strong acid (pH=1.5),the fluorescence of SAR、NAR and MAR has became weak,the cellular shape has changed,as the acidity has fallen,the fluorescence has increased,and the cellular shape has changed less,so the acid rain solution can hurt the Tobacco BY-2 cells so depply,and the cell activity has fallen and make cells dead.5. Simulate different types acid rain, the DNA of Tobacco BY-2 cells has under the influence, the strong acid rain(pH=1.5),the DNA has reduced,so it can hurt the DNA and the cells.It has influence on genetic material.