Research On The Extraction And Concentration Technique Of Saponin From Xanthoceras Sorbifolia And The Purification Technique Of Xanthoceraside

According to historical records, Xanthoceras sorbifolia Bunge.(Sapindaceae) is a kind of oil-producing tree particularly growing in China. The original birthplace of Xanthoceras sorbifolia is northern China. To a certain extent, Xanthoceras sorbifolia has been found some functions of anti-inflammation, anti-tumor, anti-oxidation and improving memory, etc. It’s no doubt that the significance of its application is great. However, there is still a long way to go for the key processing technique. The thesis aims to provide with some practical support for the industrialization of Xanthoceras sorbifolia which is based on the optimization experiments of the extraction technique of saponin and the purification of xanthoceraside along with the application of defoamer.1. We established a method to determine the quantity of total saponins in ethanol extacts by using UV-visa spectrum. Oleanlicacid was used as contrast and the detecting wave length was at 537 nm. We used 8% vanillin-glacial acetic acid solution and 72% H2SO40.5mL,5mL, respectively. The temperature and time of water bath was 60℃ and 15min,respectively.2. We set up a method to determine the quantity of xanthoceraside. The Chromsphere YMC-Pack ODS-A column was used. The mobile phase was acetonitrile (30%-70%). The flow rate was 0.53mL/min. Column temperature was 30℃ and the detection wavelength was 225nm.3. We extracted the saponin from Xanthoceras sorbifolia Bunge shell with the method of ultrasound-assisted extraction. The effects of materials liquid ratio (ML), ultrasound temperature (UTe), ultrasound time (UTi) and ultrasound intensity (UI) on extraction rate of crude extract were studied and the optimize extraction conditions were done by orthogonal experiment. The optimize extraction conditions were that the UTe, UI, UTi, SR was 70 degrees,180w,30minutes,1:25, respectively; the optimize extraction rate was 17.26%.4. The defoamer was used in concentration process of total saponins. The optimize conditions were that the temperature of concentration was 70 ℃, the concentration of defoamer was 0.10g/mL, the sample:defoamer was 6.25:1. The surface tension and defoamer efficiency was 14.32mN/m,43.14%, respectively.5. Then the xanthoceraside was separated and purified through silica gel H and ODS-A column. The optimize conditions were that the mobile phase was methanol-ethyl acetate solution, the flow rate was 60 drips/min and the purity was 86.78%.